Open Access Research article

Schmidtea mediterranea phylogeography: an old species surviving on a few Mediterranean islands?

Eva M Lázaro1, Abdul Halim Harrath23, Giacinta A Stocchino4, Maria Pala4, Jaume Baguñà1 and Marta Riutort1*

Author Affiliations

1 Institut de Recerca de la Biodiversitat and Dept. Genètica, Facultat de Biologia, Universitat de Barcelona, Av Diagonal, 645, Barcelona 08028, Spain

2 Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia

3 Laboratoire de Biologie de la Reproduction et du Développement Animal, Département de Biologie, Faculté des Sciences de Tunis, Université de Tunis El-Manar, Campus Universitaire, 2092 El Manar, Tunis, Tunisia

4 Dipartimento di Zoologia e Genetica Evoluzionistica, dell'Università di Sassari, Corso Margherita di Savoia 15, 07100 Sassari, Italy

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BMC Evolutionary Biology 2011, 11:274  doi:10.1186/1471-2148-11-274

Published: 26 September 2011

Additional files

Additional file 1:

Species used in the first dating tree and their GenBank Accession Numbers.

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Additional file 2:

Netrin haplotypes obtained in the cloning process for each individual.

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Additional file 3:

Results of the SAMOVA analysis. A, W vs. C and S populations; B, W and C vs. S populations; C, W vs. C vs. S populations; D, W vs. C vs. TUN_LEB vs. Sicilian populations; E, W vs. S vs. SAR_SIL vs. C populations, except SAR_SIL. The percentage of variation among groups greatly increased in COI and CYB from 2 to 3 groups but remains almost unchanged from 3 to 4. Additionally, the percentage of variation among populations within groups greatly decreased in the three genes from 2 to 3 but decreased very little from 3 to 4.

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Additional file 4:

Ultrametric tree obtained with BEAST to find the age of the split between S. mediterranea and S. polychroa. Numbers in nodes represent the posterior probability/bootstrap values obtained in the phylogenetic analyses performed with MrBayes and PHYML (not shown). * indicates the maximum value, and the symbol - indicates values <0.5 or 50%. Purple numbers represent the confidence interval (95%) for the age of the node. Coloured branches indicate the membership of S. mediterranea individuals to one of the geographical groups: W in red, C in blue and S in green.

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Additional file 5:

The effect of asexual individuals on the dating analyses.

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