Open Access Research article

Genomic, RNA, and ecological divergences of the Revolver transposon-like multi-gene family in Triticeae

Motonori Tomita1*, Asuka Okutani1, Avigdor Beiles2 and Eviatar Nevo2

Author Affiliations

1 Molecular Genetics Laboratory, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan

2 Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel

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BMC Evolutionary Biology 2011, 11:269  doi:10.1186/1471-2148-11-269

Published: 25 September 2011

Additional files

Additional file 1:

Three classes of Revolver mRNA. Revolver cDNAs obtained by RT-PCR, total lengths of 665 to 723 bp, were classified into three subfamilies wherein the regions of the second and third exons were almost identical, while the region of the first exon exhibited a low homology of 60% among the families because of duplication or deletion. Repetitive sequence units composed of 8 to 14 bp are present in the same direction in the first exon as seen on the dot plot. Neighbor-joining tree of Revolver cDNA sequences in the Triticeae indicated alongside species names showed major three clusters according to the three sub-families; numbers on branches indicate the boot strap values and homologies.

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Additional file 2:

Sequence alignment between authentic Revolver genomic clones from rye IR27 (Revolver-3, 5, 6: AB124641, 124643, 124644) and newly isolated Revolver genomic clones (Revolver-3 on Chr. 6R, Revolver-5 on Chr. 1R, Revolver-6 on Chr. 5R: AB646252-646254) from each rye Imperial chromosome added in wheat. Each genomic clone localized on the chromosomes exhibits homology of 96~98% between the genomes of IR27 and Imperial.

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Additional file 3:

Geographic distribution of 18 tested populations of wild emmer wheat at 15 sites in Israel. Populations 1-6 were collected from warm, semi-humid environments on the Golan Plateau and near the Sea of Galilee (Yehudiyya, Gamla, Ammiad, Rosh-Pinna, Tabigha, and Mt. Hermon). Populations 7-11 were collected across a wide geographic and marginal steppic area on northern, eastern, and southern borders of wild emmer distribution involving hot, cold, and xeric peripheries (Mt. Gilboa, Mt. Gerizim, Gitit, Kokhav-Hashahar, and J'aba). Populations 12-15 were collected from marginal Mediterranean areas, which are the humid western borders of wild emmer distribution (Amirim, Bet Oren, Bat Shelomo, and Giv'at Koah).

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Additional file 4:

Copy numbers of Revolver and geographic and climatologic data for 18 populations of Triticum dicoccoides in Israel.

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