Schematic diagram of Adh and LEAFY genes. Open boxes represent exons, and connecting lines represent introns. Combinations of neighboring exons show the loss of intron 4 in Adh2-2 and the loss of intron 7 in Adh1-1 and Adh1-2. Arrows indicate the locations and directions of primers used for PCR amplification. Rows a, b and c are different Adh genes from Malus and Pyrus obtained in this study; Row d is the genomic Adh sequence from Fragaria (X15588); Row e is Adh1-F from Zea (AF050457); Row f is LEAFY from Malus and Pyrus. For Rows b and c, the exon 1 and intron 1of Adh2-1 and Adh2-2 were not amplified by primers Adh2-F and Adh2-R, and the empty boxes indicate incomplete exon 2 and exon 10.
Zheng et al. BMC Evolutionary Biology 2011 11:255 doi:10.1186/1471-2148-11-255