Comparative qPCR analysis and codon usage bias of A. willeyana and prokaryotic genes. The bar chart illustrates the expression levels of six A. willeyana house keeping genes quantified relative to Awi-Spherulin. Error bars are the standard error of 3 replicates derived from 3 individuals. The scatter plot is a correspondence analysis of relative synonymous codon usage (RSCU) of 181 A. willeyana genes. Superimposed are the RSCUs of 10 prokaryotic Spherulin homologs (grey circles) and Awi-Spherulin (red circle). Also indicated by color are the genes used in the qPCR analysis.
Jackson et al. BMC Evolutionary Biology 2011 11:238 doi:10.1186/1471-2148-11-238