A. willeyana and characterization of Awi-Spherulin expression. (A) Lateral and (B), apical views of A. willeyana. (C) Expanded view of the boxed region in (B) illustrates nascent spherulites. (D), SDS-PAGE gel of organic material extracted from purified spherulites. The band at 52 kDa (arrow) is Awi-Spherulin. Bands indicated by asterisks are Astrosclerin. (E) Apical view similar to that in (C) following WMISH against Awi-Spherulin. Positive cells have a distribution indicative of a role in spherulite formation. (F-H) Sections of WMISH preparations show Awi-Spherulin is expressed within spherulite forming cells. (I-K) WMISH against Astrosclerin reveals the same expression pattern as Awi-Spherulin. Arrowhead in (I) indicates the insoluble organic matrix of an individual spherulite.
Jackson et al. BMC Evolutionary Biology 2011 11:238 doi:10.1186/1471-2148-11-238