Open Access Highly Accessed Research article

A horizontal gene transfer supported the evolution of an early metazoan biomineralization strategy

Daniel J Jackson1*, Luciana Macis1, Joachim Reitner1 and Gert Wörheide23

Author Affiliations

1 Courant Research Centre Geobiology, Georg-August-University of Göttingen, Goldschmidtstr. 3, 37077 Göttingen, Germany

2 Department of Earth- and Environmental Sciences & GeoBioCenterLMU, Ludwig-Maximilians-Universität München, Richard-Wagner-Str. 10, 80333 München, Germany

3 Bavarian State Collections of Palaeontology & Geology, Richard-Wagner-Str. 10, 80333 München, Germany

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BMC Evolutionary Biology 2011, 11:238  doi:10.1186/1471-2148-11-238

Published: 12 August 2011

Additional files

Additional file 1:

The full-length sequence of Awi-Spherulin. The full-length sequence of Awi-Spherulin with annotations. Coding DNA sequence is uppercase. The signal sequence is highlighted grey, a 940 bp intron is highlighted red, a polypyrimidine tract is highlighted in white, two putative polyadenylation signals are boxed and the peptide recovered by Edman sequencing is highlighted green.

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Open Data

Additional file 2:

A list of the Eukaryotic genomes exhaustively searched by BLAST for Spherulin homologs. The following draft genome sequences were searched for the presence of Spherulin homologs using NCBI's genomic node-based BLAST tool.

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Additional file 3:

The A. willeyana genes used to asses codon usage bias. These genes were collected from an in-house EST dataset and were used to establish the typical codon usage spread of A. willeyana genes. Against this background, the codon usage biases of Awi-Spherulin and Spherulin homologs form prokaryotes was plotted using CodonW.

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Additional file 4:

Primer sequences. A list of primer sequences used to amplify the genes investigated in this study.

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Additional file 5:

Alignment of Spherulin homologs. An alignment in FASTA format of Spherulin homologs from A. willeyana, A. queenslandica and 7 proteobacteria generated by ClustalW2 with gap opening and gap extension penalties set to 3 and 1.8 respectively.

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