GPRC5 receptor family in fish. (A) Phylogenetic tree of all GPRC5B and GPRC5C sequences found in fish showing the separation of GPRC5Ba/GPRC5Bb, and GPRC5Ca/GPRC5Cb. Branch labels indicate posterior probabilities. Branch lengths represent phylogenetic distances. The burnin value for the Bayesian analysis was 500. (B) PCR with degenerate primers for amplification of GPRC5Ca and GPRC5Cb. O. latipes and P. reticulata showed a GPRC5Cb amplicon, whereas D. rerio and R. espei did not. Marker (250 bp) is indicated with M.
Kurtenbach et al. BMC Evolutionary Biology 2011 11:234 doi:10.1186/1471-2148-11-234