Phylogenetic analysis and conserved expression of GPRC5 receptors. (A) Phylogenetic tree obtained by Bayesian analysis, branch labels indicate posterior probabilities and ML-bootstrap values. (B) Amplification of GPRC5A-C in lung and brain from chicken (Gallus gallus) by RT-PCR. Beta-actin (Actb) was used as positive control, mRNA without RT as negative control. (C) Amplification of GPRC5A/D, GPRC5Ba and GPRC5Bb in skin, spinal cord, intestines, muscle, eye, gills, lung and brain from fish (Oryzias latipes) by RT-PCR. Beta-actin (Actb) was used as positive control.
Kurtenbach et al. BMC Evolutionary Biology 2011 11:234 doi:10.1186/1471-2148-11-234