Email updates

Keep up to date with the latest news and content from BMC Evolutionary Biology and BioMed Central.

Open Access Research article

Early evolution of the biotin-dependent carboxylase family

Jonathan Lombard and David Moreira*

Author Affiliations

Unité d'Ecologie, Systématique et Evolution, UMR CNRS 8079, Univ. Paris-Sud, 91405 Orsay Cedex, France

For all author emails, please log on.

BMC Evolutionary Biology 2011, 11:232  doi:10.1186/1471-2148-11-232

Published: 9 August 2011

Additional files

Additional file 1:

Schematic phylogenetic trees of biotin carboxylase (BC) sequences and CoA-substrate related carboxyl transferase (CCT) sequences. These preliminary FastTree reconstructions allowed the functional assignment of each sequence according to a phylogenetic framework and functional data from the available literature.

Format: PDF Size: 142KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Biotin-dependent carboxylase content in bacterial complete genome sequences. This is an Excel file showing the presence or absence of homologues of the different biotin-dependent carboxylases in a collection of complete genome sequences.

Format: XLS Size: 99KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Alternative evolutionary scenarios for the early diversification of the biotin-dependent carboxylases. Colored blocks represent functional protein domains. The first scenario postulates the duplication of an ancient biotin-dependent carboxylase and the insertion of a pyruvate carboxylase carboxyl transferase (PCT) within the BT/PT domain predated the cenancestor. This organism had a polypeptidic PYC and a two-subunit XCC that evolved through several duplications and splits in Archaea and Bacteria. The second scenario explores the possibility that the cenancestor bore a CCT divided in two subunits as in contemporary bacterial ACC.

Format: PDF Size: 148KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

List of all complete genome sequences used in this work. This is a text file containing the complete list of species upon which we carried out sequence similarity searches to detect biotin-dependent carboxylase homologues.

Format: PDF Size: 38KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5:

Masked multiple sequence alignment of biotin carboxylase (BC) domain sequences (NEXUS format). This alignment was used to reconstruct the tree shown in Figure 3.

Format: TXT Size: 118KB Download file

Open Data

Additional file 6:

Masked multiple sequence alignment of archaeal and bacterial biotin carboxylase (BC) domain sequences (NEXUS format). This alignment was used to reconstruct the tree shown in Figure 4.

Format: TXT Size: 70KB Download file

Open Data

Additional file 7:

Masked multiple sequence alignment of CoA-substrate related carboxyl tranferase (CCT) domain sequences (NEXUS format). This alignment was used to reconstruct the tree shown in Figure 5.

Format: TXT Size: 84KB Download file

Open Data

Additional file 8:

Masked multiple sequence alignment of pyruvate carboxylase carboxyl tranferase (PCT) domain sequences (NEXUS format). This alignment was used to reconstruct the tree shown in Figure 6.

Format: TXT Size: 58KB Download file

Open Data

Additional file 9:

Masked multiple sequence alignment of biotin carboxyl ligase (BPL) sequences (NEXUS format). This alignment was used to reconstruct the tree shown in Figure 7.

Format: TXT Size: 32KB Download file

Open Data