Open Access Highly Accessed Research article

Molecular adaptation in flowering and symbiotic recognition pathways: insights from patterns of polymorphism in the legume Medicago truncatula

Stéphane De Mita1, Nathalie Chantret2, Karine Loridon2, Joëlle Ronfort2 and Thomas Bataillon34*

Author Affiliations

1 UMR Diversité, Adaptation et Développement des Plantes Cultivées (DIADE), Institut de Recherche pour le Développement (IRD), Montpellier, France

2 UMR Amélioration Génétique et Adaptation des Plantes Méditerranéennes et Tropicales (AGAP), Institut National de la Recherche Agronomique (INRA), Montpellier, France

3 Bioinformatics Research Center, Aarhus University, Denmark

4 Institute of Biology, Aarhus University, Denmark

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BMC Evolutionary Biology 2011, 11:229  doi:10.1186/1471-2148-11-229

Published: 1 August 2011

Additional files

Additional file 1:

Table S1 - List of Medicago truncatula accessions. Microsoft Word document containing the list of Medicago truncatula accessions that were re-sequenced in this study, their accession number, country of origin and sample information.

Format: DOC Size: 46KB Download file

This file can be viewed with: Microsoft Word Viewer

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Additional file 2:

Table S2 - PCR primers. Microsoft Excel spreadsheet containing the list of PCR primers used to amplify and sequence flowering and symbiotic gene fragments.

Format: XLSX Size: 8KB Download file

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Additional file 3:

Table S3 - List of sequenced loci. Microsoft Excel spreadsheet containing the list of sequenced loci, genomic location and results of sequencing. la is the alignment length and ls is the length of the sequenced obtained from A17 with alignment gap stripped.

Format: XLS Size: 12KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 4:

Table S4 - Standard diversity indices for each fragment. Microsoft Excel spreadsheet containing standard diversity indices computed for each fragment. The results of different summary statistics are presented in separate sub-tables for control fragments, flowering loci and nodulation loci. NC: not computed.

Format: XLS Size: 21KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 5:

Figure S1 - Likelihood profile for K, the number of groups in the sample. Microsoft Word document showing the likelihood profile for K in the structure analysis. Likelihood was obtained using the software Instruct on the nucleotide polymorphism data from control loci.

Format: DOC Size: 14KB Download file

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Additional file 6:

Figure S2 - Group membership of the accession in structure analysis. Microsoft Powerpoint presentation containing the schematic representation of the assignment of the accessions in each group following Instruct analysis for K = 2, K = 3 and K = 4.

Format: PPT Size: 169KB Download file

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Additional file 7:

Table S5 - Standard diversity indices for each fragments within both ancestry groups. Microsoft Excel spreadsheet containing the values of standard diversity indices estimated for each fragment within both ancestry groups. NC: not computed.

Format: XLS Size: 32KB Download file

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Additional file 8:

Table S6 - P-values of the joint (D, Z) test. Microsoft Excel spreadsheet containing, for each locus, the value of D, Z, and the p-value for each group (P1 and P2 are the two p-values for a given locus in group 1 and group 2 respectively).

Format: XLSX Size: 10KB Download file

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