Additional file 4.

An image of an agarose gel with the five main patterns (C1, C1+C2, C2, C2+B, and B) and the five minor patterns of specimens with D. galeata mtDNA in Hyg5, Fsm25, and Myg29, and specimens with D. dentifera mtDNA in Amr43 and Ngn9. The patterns were obtained by digesting the nuclear ITS-2 region with the RsaI restriction enzyme. The last lane is a size ladder with rungs from 300 bp to 1000 bp.

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Ishida et al. BMC Evolutionary Biology 2011 11:209   doi:10.1186/1471-2148-11-209