Chromosome painting with the HMA-derived Hm-X1 (green) and EER-derived Ee-Y (red) probes. The probes hybridized to the female and male metaphase chromosomes in Hoplias malabaricus (HMA) karyomorphs C (2n = 40, XX/XY) and D (2n = 40/39, X1X1X2X2/X1X2Y). Note that the Hm-X1 probe hybridized completely to the poorly differentiated X and Y chromosomes in karyomorph C, as well as the X1 chromosome and a large extension of the neo-Y chromosome in karyomorph D. The EER-derived Ee-Y probe entirely labeled two distinct submetacentric autosomal pairs of HMA chromosomes. Scale bar = 5 μm.
Cioffi et al. BMC Evolutionary Biology 2011 11:186 doi:10.1186/1471-2148-11-186