Localization of positively selected genes within the cephalopod camera eye. (A, B) Whole-mount in situ hybridization with probes for the centaurin gamma homolog. (C) Control experiment using whole-mount in situ hybridization with sense probes for the centaurin gamma homolog. (A) The centaurin gamma transcripts were detected in the optic lobes (OL), but not in the mantle (M) or eyes (E) at stage 22. (B) Specific staining was found in the head region, including the optic lobes (OL), at stage 25. Non-specific staining was detected in the shell sac (S), as shown in (C). (C) Non-specific staining was detected in the shell sac, but not in the head region. (D) A horizontal cryosection at the solid line in (B). The Centaurin gamma transcripts appears to be restricted in the perikaryal part of the central brain and optic lobes (OL). (A), (C), stage 22. (B), stage 25. Bar = 100 μm in (A-C); 50 μm in (D).
Yoshida and Ogura BMC Evolutionary Biology 2011 11:180 doi:10.1186/1471-2148-11-180