Open Access Research article

The two tryptophans of β2-microglobulin have distinct roles in function and folding and might represent two independent responses to evolutionary pressure

Sara Raimondi1, Nicola Barbarini2, Palma Mangione1, Gennaro Esposito3, Stefano Ricagno4, Martino Bolognesi4, Irene Zorzoli5, Loredana Marchese1, Cristina Soria1, Riccardo Bellazzi2, Maria Monti6, Monica Stoppini1, Mario Stefanelli2, Paolo Magni2 and Vittorio Bellotti1*

  • * Corresponding author: Vittorio Bellotti vbellot@unipv.it

  • † Equal contributors

Author Affiliations

1 Department of Biochemistry, University of Pavia, via Taramelli 3b, 27100 Pavia, Italy

2 Department of Computer Engineering and Systems Science, University of Pavia, via Ferrata 1, 27100 Pavia, Italy

3 Department of Biomedical Sciences and Technologies, University of Udine, P.le Kolbe 4, 33100 Udine, Italy

4 Department of Biomolecular Sciences and Biotechnology, CNR-INFM and CIMAINA, University of Milano, Via Celoria 26, 20133-Milan, Italy

5 Department of Internal Medicine and Medical Therapy, University of Pavia, viale Golgi 19, 27100 Pavia, Italy

6 Department of Organic Chemistry and Biochemistry, University of Naples "Federico II" and CEINGE-Biotecnologie Avanzate, via Cinthia 6, 80126 Naples, Italy

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BMC Evolutionary Biology 2011, 11:159  doi:10.1186/1471-2148-11-159

Published: 10 June 2011

Additional files

Additional file 1:

Reconciliation between reduced species tree and reduced gene tree, conducted by GeneTree 1.3.0. Good topology agreement is demonstrated between the reduced versions of the species and gene trees.

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Additional file 2:

Reconciled tree for the reduced β2-microglobulin gene family. The reduced gene tree was reconciled using GeneTree with the species tree. Squares indicate duplication events, grey lines indicate absent genes, either lost from those species or not yet sequenced.

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Open Data

Additional file 3:

Reconciled tree for the whole β2-microglobulin gene family. The gene tree was reconciled with the species tree using Notung 2.6 (parameter values: 0.9 for losses, 1.35 for duplications, and no cost for conditional duplications). The D/L Score has been used to infer the root of a gene tree. Red squares indicate duplication events, grey lines indicate absent genes, either lost from those species or not yet sequenced. Edges with the minimum root score are highlighted in red and edges with near optimal scores are in pink.

Format: PNG Size: 149KB Download file

Open Data