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Open Access Research article

Molecular evolution of the crustacean hyperglycemic hormone family in ecdysozoans

Nicolas Montagné1, Yves Desdevises2, Daniel Soyez3 and Jean-Yves Toullec4*

Author Affiliations

1 UPMC Univ Paris 06, UMR A 1272 INRA - Physiologie de l'Insecte: Signalisation et Communication, F-75005, Paris, France

2 UPMC Univ Paris 06, FRE 3247 CNRS - Modèles en Biologie Cellulaire et Évolutive, Observatoire Océanologique, F-66651, Banyuls-sur-Mer, France

3 UPMC Univ Paris 06, ER3 - Biogenèse des Signaux Peptidiques, F-75005, Paris, France

4 UPMC Univ Paris 06, UMR 7144 CNRS - Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, F-29682, Roscoff, France

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BMC Evolutionary Biology 2010, 10:62  doi:10.1186/1471-2148-10-62

Published: 25 February 2010

Abstract

Background

Crustacean Hyperglycemic Hormone (CHH) family peptides are neurohormones known to regulate several important functions in decapod crustaceans such as ionic and energetic metabolism, molting and reproduction. The structural conservation of these peptides, together with the variety of functions they display, led us to investigate their evolutionary history. CHH family peptides exist in insects (Ion Transport Peptides) and may be present in all ecdysozoans as well. In order to extend the evolutionary study to the entire family, CHH family peptides were thus searched in taxa outside decapods, where they have been, to date, poorly investigated.

Results

CHH family peptides were characterized by molecular cloning in a branchiopod crustacean, Daphnia magna, and in a collembolan, Folsomia candida. Genes encoding such peptides were also rebuilt in silico from genomic sequences of another branchiopod, a chelicerate and two nematodes. These sequences were included in updated datasets to build phylogenies of the CHH family in pancrustaceans. These phylogenies suggest that peptides found in Branchiopoda and Collembola are more closely related to insect ITPs than to crustacean CHHs. Datasets were also used to support a phylogenetic hypothesis about pancrustacean relationships, which, in addition to gene structures, allowed us to propose two evolutionary scenarios of this multigenic family in ecdysozoans.

Conclusions

Evolutionary scenarios suggest that CHH family genes of ecdysozoans originate from an ancestral two-exon gene, and genes of arthropods from a three-exon one. In malacostracans, the evolution of the CHH family has involved several duplication, insertion or deletion events, leading to neuropeptides with a wide variety of functions, as observed in decapods. This family could thus constitute a promising model to investigate the links between gene duplications and functional divergence.