Research article
Lineage-specific positive selection at the merozoite surface protein 1 (msp1) locus of Plasmodium vivax and related simian malaria parasites
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* Corresponding author: Kazuyuki Tanabe kztanabe@biken.osaka-u.ac.jp
1 Laboratory of Malariology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan
2 Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan
3 Bioinformatics and Molecular Evolution, University of Vigo, Vigo 36310, Spain
4 Malaria Research Unit, Department of Parasitology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka
5 Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka
6 Center for Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi 321-0293, Japan
7 Graduate School of Agriculture and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan
BMC Evolutionary Biology 2010, 10:52 doi:10.1186/1471-2148-10-52
Published: 19 February 2010Additional files
Additional file 1:
Malaria parasite species and isolates used in the present study. Table showing malaria parasite species and isolates as well as accession numbers of sequences obtained in this study and retrieved from the GenBank database.
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Additional file 2:
Predicted molecular weight of MSP-1 of P. vivax and P. vivax-related simian malaria parasites. Table showing variation in molecular weights and amino acid lengths of MSP-1 among P. vivax and P. vivax-related simian malaria parasites and within several parasite species.
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Additional file 3:
Amino acid sequence alignment of MSP-1 from P. vivax and P. vivax-related simian malaria parasite species. Figure S1 showing amino acid alignment of MSP-1 from P. gonderi, P. fragile, P. coatneyni, P. knowlesi, P. hylobati, P. inui, P. fieldi, P. vivax, and P. cynomolgi. This figure contains information of sequence regions of four inter-species variable blocks and those used for constructing phylogenetic trees and amino acid sites under positive selection.
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Additional file 4:
Phylogenetic trees for estimating divergence times among P. vivax and P. vivax-related simian malaria parasite lineages. Figure S2 showing a method for phylogeny-based estimation of divergence times among P. vivax and P. vivax-related simian malaria parasite lineages.
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Additional file 5:
Positional overlap of positively selected amino acid regions of MSP-1 from P. vivax, P. inui and P. cynomolgi. Figure S3 showing five overlapping positively selected amino acid sequence regions of P. vivax, P. inui and P. cynomolgi that were inferred by the omegaMap.
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Additional file 6:
Indels and repeats in a variable block (VB4) of msp1 from P. inui. Figure S4 showing a highly variable sequences due to indels and repetitive sequences in a variable block (VB4) of P. inui msp1.
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