Table 3

Primers used for PCR amplification and sequencing

Marker

Primer name

Purpose

Sequence

Reference


ITS2 (nuclear)

ITSc2-5

PCR + sequencing

5'-AGCCAGCTGCGATAAGTAGTG-3'

[42]


ITS2 (nuclear)

R28S1

PCR + sequencing

5'-GCTGCAATCCCAAACAACCC-3'

[42]


ATPSβ (nuclear)

ATPSβf5

PCR

5'-CCAAGGGTGGNAARATHGGT-3'

this article


ATPSβ (nuclear)

ATPSβr2

PCR + sequencing

5'-GGTTCGTTCATCTGACCATACAC-3'

[26]


ATPSβ (nuclear)

ATPSβf2

sequencing

5'-TGAAAGACAAGAGCTCCAAGGTA-3'

[26]


ATPSβ (nuclear)

ATPSβf4

sequencing

5'-GAGCTGGTGTTGGAAAGACTGT-3'

this article


ORF (mitochondrial)

FATP6.1

PCR + sequencing

5'-TTTGGGSATTCGTTTAGCAG-3'

[26]


ORF (mitochondrial)

RORF

PCR + sequencing

5'-SCCAATATGTTAAACASCATGTCA-3'

[26]


ORF (mitochondrial)

FORF

sequencing

5'-GTGCGCCAGCATTCTATTG-3'

this article


ORF (mitochondrial)

RORF2

sequencing

5'-TAGAATGCTGGCGCACATAA-3'

this article


CR (mitochondrial)

FNAD5.2deg

PCR + sequencing

5'-GCCYAGRGGTGTTGTTCAAT-3'

[26]


CR (mitochondrial)

RCOI3deg

PCR + sequencing

5'-CGCAGAAAGCTCBARTCGTA-3'

[54]


CR (mitochondrial)

FNC1

sequencing

5'-GGGGTGAGATGAAGAGGTGA-3'

this article


CR (mitochondrial)

RNC1

sequencing

5'-CGGGTGCCACTATGTTTTCT-3'

this article


Flot et al. BMC Evolutionary Biology 2010 10:372   doi:10.1186/1471-2148-10-372

Open Data