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Open Access Highly Accessed Research article

Ancient origin of a Western Mediterranean radiation of subterranean beetles

Ignacio Ribera12*, Javier Fresneda3, Ruxandra Bucur45, Ana Izquierdo1, Alfried P Vogler45, Jose M Salgado6 and Alexandra Cieslak1

Author Affiliations

1 Museo Nacional de Ciencias Naturales, José Gutiérrez Abascal 2, 28006 Madrid, Spain

2 Institute of Evolutionary Biology (CSIC-UPF), Passeig Maritim de la Barceloneta 37-49, 08003 Barcelona, Spain

3 Ca de Massa, E-25526 Llesp, Lleida, Spain

4 Natural History Museum, Cromwell Road, London SW7 5BD, UK

5 Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK

6 Departamento de Biología Animal, Facultad de Biología, Universidad de León, León, Spain

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BMC Evolutionary Biology 2010, 10:29  doi:10.1186/1471-2148-10-29

Published: 28 January 2010

Additional files

Additional file 1:

Specimens used in the study, with locality, collector, voucher reference numbers and accession numbers for the sequences. In grey, specimens combined in a composite sequence.

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Additional file 2:

Estimation of the parameters for the Bayesian analyses.

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Additional file 3:

Cladogram obtained with MrBayes with the MAFFT alignment including the Sardinian species, with detailed node support values. Upper row, MrBayes posterior probabilities, MF/PR; lower row, bootstrap support values (1,000 replicas) in Garli, MF/PR.

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Additional file 4:

Phylogram obtained with MrBayes with the MAFFT alignment, with the exclusion of the Sardinian species.

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Additional file 5:

Ultrametric tree obtained with Beast using the cox1 sequence only, including the Sardinian species (red clade). Black circles, well supported nodes (see Fig. 1, Additional file 3) constrained to be monophyletic. Numbers inside nodes, age estimate (MY) using the separation of the Sardinian species with a prior age of 33MY (see text).

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Additional file 6:

Age estimations of the nodes in Fig.3and Additional file5. Vertical axis, estimated age (MY). Horizontal axis, nodes. White circles, estimation using the combined mitochondrial genes (cox1, rrnL+trnL, nad1, cob) in Beast, with 95% confidence intervals (dashed lines) (Fig. 3). Black circles, estimation using only cox1 in Beast, with 95% confidence interval (solid line) (Additional file 5). Red circles, estimation using the gen cox1 in r8s. Estimations using cox1 alone were calibrated with the node "Sardinian clade [cox1]", and those using the combined mtDNA with the node "Sardinian clade [mt]". Note than in the estimation using the combined mtDNA genes the Sardinian species were not included.

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