Additional file 2.

A multiple alignment of amino acid sequences deduced from teleostean hatching enzyme genes. All hatching enzymes were composed of a signal sequence (putative cleavage sites are shown as white triangle), a pro-sequence, and a mature enzyme sequence (the N-terminals are shown as black triangle). The mature enzyme portion possesses two active site consensus sequences for astacin family metallo-proteases, HExxHxxGFxHExxRxDR (Zn-binding site, indicated in dark gray) and SxMHY (methionine turn, indicated in light gray). In addition, six conserved cysteine residues are shaded in black. Red lines indicate the intron insertion sites. Identical residues are boxed. Dashes, asterisks and "X"s represent gaps, stop codons and unidentified amino acid residues, respectively. Arrows at the bottom indicate sites of primers designed for amplification of hatching enzyme gene fragments.

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Kawaguchi et al. BMC Evolutionary Biology 2010 10:260   doi:10.1186/1471-2148-10-260