Research article
Frequent gene conversion events between the X and Y homologous chromosomal regions in primates
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* Corresponding author: Mineyo Iwase iwase_mineyo@soken.ac.jp
1 The Center for the Promotion of Integrated Sciences, The Graduate University for Advanced Studies (Sokendai), Shonan village, Hayama, Kanagawa 240-0193, Japan
2 Department of Evolutionary Studies of Biosystems, The Graduate University for Advanced Studies (Sokendai), Shonan village, Hayama, Kanagawa 240-0193, Japan
3 Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan
4 The Graduate University for Advanced Studies (Sokendai), Shonan village, Hayama, Kanagawa 240-0193, Japan
BMC Evolutionary Biology 2010, 10:225 doi:10.1186/1471-2148-10-225
Published: 23 July 2010Additional files
Additional file 1:
Window analysis of nucleotide differences (y-axis) along the X chromosome. The position of the KALX and VCX exons are indicated at the bottom of each graph. Each bar corresponds to a p-distance in a non-overlapping window of 500 bp. A low p-distance region is indicated by the two-headed arrows. The region indicated by the rectangle is enlarged in Figure 1B.
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Additional file 2:
FISH analysis using species-specific KALX and KALY probes. FISH was carried out on metaphase chromosomes from human (a, b, c and d), chimpanzee (e, f, g and h), and rhesus monkey (i, j, k and l). The X (left: a, b, e, f, i. j) and Y (Right; c, d, g, h, k, l) chromosomes are marked (arrowheads). Green fluorescence indicates a positive signal. A short white bar marks the centromeric region (scale bar: 10 μm).
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Additional file 3:
The phylogenetic tree of region A and B of KALX and KALY in several primates. The trees for Regions A and B are based on p-distances. The numbers of sites are given at the bottom right of each tree. A yellow diamond at the node indicates KALX and KALY differentiation, and the number beside each node is the bootstrap probability after 500 replications.
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Additional file 4:
The phylogenetic tree of VCX and VCY in several primates. Diamonds indicate gene conversion events.
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Additional file 5:
Sources of DNA samples and GenBank accession numbers of the analyzed sequences. All the nucleotide sequences reported in this study were deposited in DDBJ (DNA Data Bank of JAPAN) and their accession numbers are AB233497 - AB233526.
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Additional file 6:
Primer sets for PCR.
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