Table 2

Biacore analysis of BALB and CAST IL4 binding to IL4Rα

k_a(1/Ms)

k_d(1/s)

K_A(1/M)

K_D(M)

BALB IL4

2.49E+06

5.18E-04

4.79E+09

2.09E-10

CAST IL4

3.16E+06

7.13E-04

4.44E+09

2.26E-10

Data are the average of two independent experiments performed with two independently coupled chips.

k_a is the association rate constant (M^-1s^-1);

k_d - is the dissociation rate constant (s^-1);

K_A - is the equilibrium binding (affinity) constant (k_a/k_d) (1/M);

K_D - is the equilibrium dissociation constant (k_d/k_a) (M)

Koyanagi et al. BMC Evolutionary Biology 2010 10:223   doi:10.1186/1471-2148-10-223