Research article
Diversifying selection and functional analysis of interleukin-4 suggests antagonism-driven evolution at receptor-binding interfaces
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* Corresponding authors: Harmit S Malik hsmalik@fhcrc.org - Mark Bix mark.bix@stjude.org
1 Department of Immunology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105 USA
2 Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, PO Box 19024, WA 98109 USA
3 HHMI, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, PO Box 19024, WA 98109 USA
4 Division of Viral Immunology, Center for Aids Research, Kumamoto University, 2-2-1, Honjo, Kumamoto 860-0811 Japan
5 Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103 USA
6 Trubion Pharmaceuticals, 2401 4th Ave, Seattle, WA 98121 USA
BMC Evolutionary Biology 2010, 10:223 doi:10.1186/1471-2148-10-223
Published: 22 July 2010Additional files
Additional file 1:
IL4 polymorphisms between BALB/c and CAST/Ei.
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Additional file 2:
Kinetic profiles of surface plasmon resonance depicting the binding of IL4 and IL4Rα.
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Additional file 3:
IL4 alignment from 28 eutherian mammals in .phy format.
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Additional file 4:
IL4 alignment from 16 primates in .phy format.
Format: TXT Size: 8KB Download file
Additional file 5:
Stages of purification of recombinant IL4 purification from insect cell supernatant as measured by functional bioassay.
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Additional file 6:
IL4 yield and fold purification.
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Additional file 7:
Monoclonal anti-IL4 antibody BVD6 binds equivalently to recombinant BALB and CAST IL4.
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