Description of the Selection Scheme. Selection was conducted independently for individuals derived from F252 and MBS in order to obtain an Early and a Late population from each original seed lot. At each generation, S1 families produced by the selfing of the 10 most early-flowering individuals (rectangles with light gray patterns) and the 10 most late-flowering individuals (rectangles with black patterns) of the previous generation are phenotypically evaluated. Hundred individuals per family were sown in a 4 randomized block design. Each block therefore encompasses 25 individuals of each family and contains individuals from the Early and the Late population, as well as a control plot (open rectangles with a dashed line). The same protocol was repeated over generations.
Durand et al. BMC Evolutionary Biology 2010 10:2 doi:10.1186/1471-2148-10-2