Email updates

Keep up to date with the latest news and content from BMC Evolutionary Biology and BioMed Central.

Open Access Highly Accessed Research article

Molecular evolution of glutamine synthetase II: Phylogenetic evidence of a non-endosymbiotic gene transfer event early in plant evolution

Sohini Ghoshroy1*, Manfred Binder1, Aurélien Tartar2 and Deborah L Robertson1

Author Affiliations

1 Clark University, Biology Department, 950, Main Street, Worcester, MA 01610, USA

2 Nova Southeastern University, 3301 College Avenue, Fort Lauderdale, FL 33314, USA

For all author emails, please log on.

BMC Evolutionary Biology 2010, 10:198  doi:10.1186/1471-2148-10-198

Published: 25 June 2010

Additional files

Additional file 1:

GSII protein sequences used in the present study. GenBank accession numbers and JGI DOE scaffold and protein ID information for the GSII proteins are provided.

Format: DOC Size: 290KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Identification of GSIIB sequences from Acetabularia acetabulum and Mesostigma viride. The data matrix used in this analyses is described in the Methods with sequences from the following taxa included in the alignment: Acetabularia acetabulum (Chlorophyta, Ulvophyceae), Mesostigma viride (Streptophyta, Mesostigmatophyceae), which are shown in bold. Pseudochlorella sp. CCAP211/1A (1) GSIIB amplified in the present study is also shown in bold. Tree used for this illustration is a representative derived from parsimony heuristic search analysis. RAxML bootstrap values are shown for the major clades of GSII genes, which were derived from 1000 bootstrap replicates with the parameters described in the Methods.

Format: PDF Size: 110KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Maximum clade probability tree displayed as a chronogram from the BEAST analysis of the GS amino acid sequence alignment. All lineages evolved according to a relaxed molecular clock and WAG + Inv. + Gamma model. Node bars indicate the width of the 95% highest posterior density with minimum and maximum values in parentheses. Bold numbers near the nodes indicate node ages. Major lineages are depicted as collapsed triangles. Nodes for which fossil dates were used are marked as A = Acsomycota, 400MYA, B = Bilateria 550MYA and C = Streptophytes 475 MYA. P = Paleozoic; M = Mesozoic; C = Cenozoic; Camb. = Cambrian.

Format: PDF Size: 44KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

Partial GSIIB sequences were identified in two additional Chloroplastida by preliminary phylogenetic analyses. Partial GSIIB sequences were obtained for Acetabularia acetabulum (Chlorophyta: Ulvophyceae) and Mesostigma viride (Streptophyta: Mesostigmatophyceae). These sequences were not included in the phylogenetic analyses presented within the paper (figures 1, 2, 3 and 4) due to their short length. Phylogenetic analysis confirming these proteins as members of the GSIIB clade is presented in Additional file 2.

Format: DOC Size: 31KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 5:

Predicted cellular localization of GSII proteins in early-diverging Chloroplastida. Only taxa containing both GSIIB and GSIIE within Chloroplastida were analyzed. N-terminal sequences of GSII proteins were analyzed for organellar transit peptides, in silico. References are given for established functional localizations.

Format: DOC Size: 76KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data