Additional file 4.

Two-dimensional Gels of β-keratin Expression in Chick Epidermal Appendages. Reprint of Figure 2 in Shames et al [19]. Two-dimensional gels of protein extracted from 19-20 day embryonic chick (A) scutate scale epidermis, (B) cornified beak, (C) egg tooth, (D) periderm, and (E) claw. The acidic (Ac) and basic (Ba) ends of the gel and the molecular weight markers are indicated for the second dimension. The protein spots labeled 1 and 2, 3 are scale β-keratins identified by hybrid-selection using a scale specific oligonucleotide probe [65].

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Greenwold and Sawyer BMC Evolutionary Biology 2010 10:148   doi:10.1186/1471-2148-10-148