Table 1

Listing of markers used to establish the identity of cortical structures in immature oocytes.

Name

Type

Target

Reference

Present in a cortical structure

CDC2

mouse monoclonal antibody

Cell division cycle 2, catalytic subunit of MPF

[22]

+

PSTAIR

mouse monoclonal antibody

Cyclin binding domain in all Cdks

[23]

+

SEC23

goat polyclonal antibody

SEC23, component of the coat protein II complex

[18]

+

P-GM130

rabbit polyclonal antibody

GM130 phosphorylated on serine 25

[27]

+

Calnexin

rabbit polyclonal antibody

Calnexin, transmembrane protein

[26]

-

Gamma-tubulin

rabbit polyclonal antibody

Gamma-tubulin, cytoskeletal component

[25]

-

Mitotracker*

Fixable live cell dye

Oxidation within mitochondria

[28]

-

NUP153

mouse monoclonal antibody

Nucleoporin 153 kDa

[29]

-

GM130

mouse monoclonal antibody

Structural Golgi protein

[27]

-

Cyclin B

mouse monoclonal antibody

Cyclin B, regulatory subunit of MPF

[22]

-

SPDY

rabbit polyclonal antibody

SPDY, regulatory protein of CDC2

[19]

+

* Stained by 30 min incubation in maturation medium prior to fixation.

Hölzenspies et al. BMC Developmental Biology 2009 9:8   doi:10.1186/1471-213X-9-8