Additional file 7.

Figure S7:. Effect of Ipiwi1 RNAi on the regeneration of I. pulchra after 21 days of Ipiwi1 dsRNA treatment. RNAi with luciferase dsRNA did not show any effect on Ipiwi1 or Ipvasa mRNA expression (A, E), Ipiwi1 protein (C) or cell proliferation (G). After 21 days of Ipiwi1 dsRNA treatment ipiwi1 expression was eliminated both on mRNA (B) as well as on protein level and only weak ipvasa mRNA could be detected in remnant eggs (F). Notably, cell proliferation was not affected up to 21 days of ipiwi1 dsRNA treatment (H). (de) developing eggs; (t) testes. Scale bars 100 μm.

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De Mulder et al. BMC Developmental Biology 2009 9:69   doi:10.1186/1471-213X-9-69