Additional file 3.

Figure S3:. Alignment of predicted piwi-like genes from I. pulchra with piwi-like genes from other species. (A) Amino acid alignment of the conserved PAZ domain. (B) Amino acid alignment of the conserved PIWI domain. The PIWI box is highlighted in purple. Amino acids indicated with green asterisks are supposed to create a binding pocket for the 5'phosphate group of binding RNA. Red asterisks indicate putative RNase active site carboxylate residues. Amino acids indicated in purple can distinguish members of the piwi and argonaute subfamily. The Genbank accession numbers: Isodiametra pulchra Ipiwi1 (AM942741); Isodiametra pulchra Ipiwi2 (AM942742); Macrostomum lignano Macpiwi (AM942740); Schmidtea mediterranea Smedwi1 (DQ186985) Smedwi2 (DQ186986); Dugesia japonica DjPiwi1 (AJ865376); Podocoryne carnea Cniwi (AAS01181); Caenorhabditis elegans PRG1 (NP492121); Drosophila melanogaster DmPiwi (AF104354); Strongylocentrotus purpuratus Seawi (AY014899); Homo sapiens Hiwi (AF104260).

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De Mulder et al. BMC Developmental Biology 2009 9:69   doi:10.1186/1471-213X-9-69