Figure 5.

Striping patterns in corneal epithelia of mosaic PAX6-GFP transgenic mice. Fluorescence microscopy of corneas of PAX6-GFP transgenic mice from lines Y223 and Y001deltaDRR (described in the Methods section) showing green fluorescence of GFP-positive tissues. A: An eye from line Y223 with bright fluorescence from the lens. B,C: Eyes from 12-month old mice from line Y001deltaDRR showing radial stripes and whorls in the cornea. The bright green fluorescence seen in the pupil in A-C is GFP fluorescence from the lens. In A it also obscures part of the pattern of stripes in the cornea whilst in B and C it is reduced due to deletion of the DRR region of the PAX6 locus. D: Histological section showing DAPI-stained nuclei (blue) and both GFP-positive (green) and GFP-negative regions in the corneal epithelium. Arrow in D indicates a GFP-positive stripe, in which GFP-positive cells are aligned vertically in the epithelium. Scale bars: A-C, 1 mm; D, 100 μm.