Figure 6.

Heterogeneity of Ngn3-GFP-expression in A_pr and A_al spermatogonia. Seminiferous tubules from adult Ngn3-GFP mice were immunostained with anti-LIN28 and anti-GFP antibodies. (A) Presence of Ngn3-GFP-negative cell(s) in A_pr and A_al (4-cell) spermatogonia. Note the unusual 4-cell chain (encircled) that is branched. (B) One cell (arrow) at the end of the 8-cell chain was Ngn3-GFP-negative. (C) Two spermatogonia (arrows) in the middle of 16-cell chain were Ngn3-GFP-negative. Scale bar, 25 μm.