Figure 3.

Results of the quantitative expression analysis of the bovine FN1 splice variants. The total FN1 RNA expression and the relative composition of each splice isoform was determined for the 3 alternatively spliced regions (A: EIIIB, B: EIIIA, C: IIICS). Oo: oocyte, 2C: in vitro 2-cell embryo, 8C: in vitro 8-cell embryo, M: in vitro morula, B: in vitro blastocyst, HB: in vitro hatched blastocyst, Cum: cumulus cells, K: kidney, Li: liver, Lu: Lung, Ud: udder, P: placenta, Sp: spleen, M: muscle, H: heart, Ut: uterus, Sk: skin, Ov: ovary. For each sample type, a t-test was performed to compared the relative expression of each splice isoform (* = P < 0.05; ** = P < 0.01).