Quantification of Fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos

doi:10.1186/1471-213X-9-1

BMC Developmental Biology

Volume 9

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Peelman LJ

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Research article

Quantification of Fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos

Karen Goossens¹ , Ann Van Soom² , Alex Van Zeveren¹ , Herman Favoreel³ and Luc J Peelman¹

¹Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium

²Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium

³Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium

author email corresponding author email

BMC Developmental Biology 2009, 9:1doi:10.1186/1471-213X-9-1


Published:	6 January 2009

Additional files

Additional file 1:

FN1 primer validation experiment. Results of the PCR amplification of each splice variant in the presence of excess of the alternative splice variant. The measuring points marked in blue indicate the standards diluted in water, while the measuring points in red represents the standards diluted in excess of the alternative variant. Both series gave equal CT values and quantities for each dilution point.

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Additional file 2:

Statistical analysis of the overall FN1 expression levels between the different sample types. An ANOVA test was used to compare the overall FN1 expression between the different sample combinations (NS: not significant P > 0.05; * = P < 0.05; ** = P < 0.01).

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Additional file 3:

RT-qPCR analysis for FN1 and Integrin subunits. Relative FN1 and Intergin mRNA expression levels in bovine oocytes and in vitro produced embryos determined by RT-qPCR. For each gene, the expression levels were compared to the highest value, set at 100%. Oo: oocyte, 2C: in vitro 2-cell, 8C: in vitro 8-cell, M: in vitro morula, B: in vitro blastocyst, HB: in vitro hatched blastocyst.

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