Pax2 expression in the medial septum does not co-localise with that of calbindin and choline acetyltransferase (Chat). A-C: Double immunohistochemistry with Pax2 (black) (A) and calbindin (magenta) (B) on P8 coronal sections reveal the presence of both calbindin-positive and Pax2-positive neurons in the septal area, but not co-expression of these proteins (C). Similarly (D-F), septal neurons that express Pax2 (green) (D) and Chat (brown) (E), do not co-express these proteins (F). The panels are high powers of the level depicted in Fig. 5C. Scale bar for all panels, 10 μm.
Fotaki et al. BMC Developmental Biology 2008 8:79 doi:10.1186/1471-213X-8-79