Reelin treatment in vitro induces a radial glial phenotype similar to Notch-1 activation. A, HNPCs were deprived of FGF and EGF for 24 hours prior to their transfection or treatment. a, HNPCs were transfected with empty vector or b NICD for 24 hours before fixation. c, HNPCs were treated with control or d, partially purified Reelin for 24 hours before fixation. The cells were later stained against GFAP. Scale bar 200 μm. B, Control and Reelin conditioned-medium were collected from HEK293 cells transfected with either control empty vector or Reelin, respectively. Reelin was partially purified (P.P) from the medium by column centrifugation and its expression was verified by western blotting using anti-reelin clone G10. C, Reelin treatment of HNPCs in vitro (1:40) induced Reelin signaling by increasing Dab-1 phosphorylation on tyrosine residue 198.
Keilani and Sugaya BMC Developmental Biology 2008 8:69 doi:10.1186/1471-213X-8-69