Figure 1.

Glycoprotein detection in the UVD seminal fluid of Spodoptera littoralis. Proteins obtained from the UVD lumen were separated by 2-D gel electrophoresis and probed with Pro-Q Emerald 300 Glycoprotein Gel Stain. Black arrows indicate most abundant glycoprotein spots with isoelectric point (pI) between 6 and 7 (numbered 1–3). These proteins were submitted to mass spectrometry analysis, which detected in proteins #1 and #2 several peptides with homology to proteins that form yolk in the oocytes.