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Open Access Research article

FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans

Jiamiao Lu12, William L Dentler1 and Erik A Lundquist1*

Author Affiliations

1 Department of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA

2 Current address : Stanford University School of Medicine, Departments of Pediatrics and Genetics, 300 Pasteur Drive, Room H310, Stanford, CA 94305-5208, USA

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BMC Developmental Biology 2008, 8:54  doi:10.1186/1471-213X-8-54

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-213X/8/54


Received:21 December 2007
Accepted:16 May 2008
Published:16 May 2008

© 2008 Lu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

In the C. elegans germ line, syncytial germ line nuclei are arranged at the cortex of the germ line as they exit mitosis and enter meiosis, forming a nucleus-free core of germ line cytoplasm called the rachis. Molecular mechanisms of rachis formation and germ line organization are not well understood.

Results

Mutations in the fli-1 gene disrupt rachis organization without affecting meiotic differentiation, a phenotype in C. elegans referred to here as the

    g
erm
    l
ine
    m
orphogenesis (Glm) phenotype. In fli-1 mutants, chains of meiotic germ nuclei spanned the rachis and were partially enveloped by invaginations of germ line plasma membrane, similar to nuclei at the cortex. Extensions of the somatic sheath cells that surround the germ line protruded deep inside the rachis and were associated with displaced nuclei in fli-1 mutants. fli-1 encodes a molecule with leucine-rich repeats and gelsolin repeats similar to Drosophila flightless 1 and human Fliih, which have been shown to act as cytoplasmic actin regulators as well as nuclear transcriptional regulators. Mutations in let-60 Ras, previously implicated in germ line development, were found to cause the Glm phenotype. Constitutively-active LET-60 partially rescued the fli-1 Glm phenotype, suggesting that LET-60 Ras and FLI-1 might act together to control germ line morphogenesis.

Conclusion

FLI-1 controls germ line morphogenesis and rachis organization, a process about which little is known at the molecular level. The LET-60 Ras GTPase might act with FLI-1 to control germ line morphogenesis.

Background

The C. elegans gonad is a bi-lobed organ composed of the germ line and somatic distal tip cells and sheath cells that partially envelop the germ line [1,2]. The distal half of the germ line is a syncytium, with multiple germ nuclei sharing a common cytoplasm. At the distal tip of the two gonad arms, germ line stem cells interact with the distal tip cell, which maintains them in a mitotic stem cell fate (the mitotic zone) [1,3]. As the nuclei proceed proximally down the germ line and lose contact with the distal tip cell niche, they exit mitosis and begin meiotic differentiation (the transition zone).

When the nuclei enter meiosis and arrest at pachytene in the meiotic zone, they are associated with the germ line cortex, resulting in a nucleus-free inner core of cytoplasm called the rachis [4,1,2]. Germ line plasma membrane invaginates between the nuclei to partially enclose them, forming a characteristic "T" structure of plasma membrane surrounding the meiotic germ nuclei [4,5]. Somatic sheath cells partially envelop the germ line and extend filopodia over bare regions, but do not extend protrusions deeply between germ line plasma membrane invaginations [4]. As pachytene nuclei reach the flexure, or bend, of the gonad arm, individual meiotic nuclei enter diakinesis and become completely enclosed by plasma membrane to complete oogenesis. Oocytes are fertilized as they move proximally through the spermatheca.

In recent years, genes and signals that control mitotic stem cell character and meiotic differentiation have been identified [6,2]. LAG-2 Delta in the distal tip and GLP-1 Notch in the germ line are required to maintain the mitotic stem cell fate in the distal tip cell niche and to repress the translation of meiotic differentiation factors [7-10]. As germ nuclei leave the niche, the meiotic differentiation factors GLD-1, 2, and 3 and NOS-1 promote meiosis and repress GLP-1 translation and the mitotic fate [11-15]. The transition zone contains a mix of mitotic and meiotic nuclei that reorganize to the cortex to form the rachis. Meiotic nuclei at the cortex arrest in pachytene until they reach the gonad flexure, where meiosis resumes and oogenesis begins. Ras/Map kinase signaling, including LET-60 Ras, is required for progression through pachytene and entry into diakinesis [16,17]. While much is known about meiotic specification, less is known about the molecular mechanisms that control rachis organization in the meiotic zone, although Ras signaling is likely involved, as mutations in let-60 Ras and mpk-1 Erk cause disorganization of the pachytene region of the germline.

Described here are initial studies showing that the fli-1 gene perturbs rachis formation without affecting meiotic progression. In fli-1 mutants, chains of germ nuclei were observed in the rachis of the meiotic zone, and ultrastructural analysis revealed that these nuclei remained associated with germ line plasma membrane. Furthermore, extensions of the sheath cells protruded into the rachis between these misplaced nuclei. This phenotype is referred to here as a

    g
erm
    l
ine
    m
orphogenesis defect (the Glm phenotype). No defects in mitotic or meiotic specification were observed in the misplaced nuclei or in any germ nuclei in fli-1 mutants.

The fli-1 locus was identified and found to encode a molecule with N-terminal leucine-rich repeats (LRRs) and 5 C-terminal gelsolin repeats, similar to the Drosophila and human Flightless 1 molecules [18,19]. C. elegans FLI-1 can bind to and sever actin filaments [20], and a fli-1 mutation caused defects in a variety of tissues, including germ line organization defects [21]. Human Flightless 1 (fliih), along with a monomer of G-actin, is a component of a transcriptional coactivator complex that acts with nuclear hormone receptors and β-catenin/TCF LEF [22,23]. In Drosophila, Flightless 1 mutants display defects in flight muscle development as well as defects in nuclear organization and cellularization in the syncytial blastoderm [19]. Thus, Flightless 1 molecules might have distinct roles in the cytoplasm and nucleus, possibly organizing the actin cytoskeleton in the former and modulating transcription in the latter.

The LET-60 Ras molecule has been shown to control meiotic progression from pachytene to diakinesis, and let-60 mutations were found to have a germ line organization defect [16]. Data presented here show that let-60 Ras has a Glm phenotype similar to fli-1, and that let-60 Ras and fli-1 interact genetically in germ line morphogenesis. Thus, FLI-1 and LET-60 Ras might act together to control germ line organization and rachis formation during meiotic differentiation in the C. elegans germline.

Results

fli-1(ky535) affects germ line morphogenesis

The ky535 mutation was isolated in a synthetic lethal screen to identify molecules that act in parallel to the actin-binding protein UNC-115 abLIM [24]. UNC-115 and FLI-1 likely have roles in pharyngeal function underlying the synthetic lethal phenotype. Pharyngeal pumping is severely reduced in unc-115; fli-1 double mutants, and double mutants arrest in the L1 larval stage consistent with a feeding defect (data not shown).

Alone, fli-1(ky535) animals were viable and fertile and displayed a slightly Dumpy (Dpy) body morphology. When observed using differential interference contrast (DIC) microscopy, germ line nuclei were observed in the rachis of the meiotic zone (compare Figures 1A and 1C to Figures 1B and 1D). In most cases, chains of apparently connected nuclei spanned the rachis. Misplaced germ cells in the rachis were observed as soon as the rachis was evident in mid-to-late L4 larval animals (data not shown). This phenotype is referred to here as the

    g
erm
    l
ine
    m
orphogenesis (Glm) phenotype. In fli-1(ky535), 94% of gonad arms displayed the Glm phenotype (Figure 2). The Glm phenotype was never observed in wild-type animals.

thumbnailFigure 1. fli-1 mutants display germ line morphogenesis defects (the Glm phenotype). In all images, the distal tip of the gonad is to the left. In images (C), (D), (E), (G), and (H), the approximate extents of mitotic zones (M), transition zones (T), and meiotic pachytene zones (P) are indicated. (A) and (B) Differential Interference Contrast (DIC) images of wild-type and fli-1(ky535) gonads. A wild-type gonad had a germ nucleus-free rachis in the meiotic zone whereas a fli-1(ky535) animal displayed chains of nuclei crossing the rachis (arrowheads in (B)). (C-E). Epifluorescence images of DAPI-stained gonads from wild-type, fli-1(ky535), and fli-1(tm362)/+ animals. Wild-type shows a nucleus-free rachis, whereas fli-1(ky535) and fli-1(tm362)/+ displayed chains of nuclei crossing the rachis (arrowheads). (F) DIC image of a gonad from an rrf-1 mutant animal subject to fli-1 RNAi (rrf-1 animals are defective for somatic RNAi but not germ line RNAi). Arrowheads indicate nuclei in the rachis. (G) and (H) Gonads from wild-type and fli-1(ky535) fed BrdU-containing bacteria for 5 minutes and fixed and stained with DAPI and anti-BrdU antibody. Nuclei in the mitotic zone of both wild-type and fli-1(ky535) accumulated BrdU. No BrdU-positive nuclei were seen in the meiotic pachytene regions, including the misplaced nuclei in fli-1(ky535). The scale bar in (A) = 10 μm for (A-H).

thumbnailFigure 2. Quantitation of the Glm phenotype in fli-1 mutants. Genotypes are indicated along the y axis, and percent of gonad arms displaying the Glm phenotype is the x axis. For each genotype, the number of gonad arms scored is indicated inside the bar, and the standard error of the proportion is shown as error bars. Asterisks indicate that the differences between genotypes are significant (p < 0.001; t-test and Fisher's Exact analysis).

Transition from mitosis to meiosis is not disrupted in fli-1(ky535) mutant germ nuclei

The misplaced nuclei in the rachis of the meiotic zone in fli-1 might have been due to disruption in the transition of nuclei from mitosis to meiosis. A BrdU incorporation was used to assay nuclei undergoing DNA synthesis in the germ line (e.g. those that have undergone mitosis or S phase of meiosis I) (see Methods) [25]. After 10 minutes of exposure to BrdU, wild-type animals displayed BrdU-positive nuclei in the distal mitotic zone (Figure 1G). fli-1(ky535)-mutant gonads displayed a similar BrdU incorporation profile (Figure 1H), and nuclei in the rachis of the meiotic zone did not incorporate BrdU. A 30-minute exposure to BrdU also resulted in no apparent differences between wild-type and fli-1(ky535) (data not shown). In sum, no differences in BrdU incorporation were detected between wild-type and fli-1(ky535), suggesting that misplaced nuclei in the rachis of the meiotic zone of fli-1(ky535) were not undergoing mitotic divisions, and that normal meiotic progression was not affected (e.g. meiosis I was not delayed). DAPI staining to assay nuclear morphology showed that misplaced nuclei in the rachis of the meiotic zone of fli-1(ky535) animals displayed a meiotic pachytene morphology; the pachytene chromosomes were individually visible with a "bowl of spaghetti" appearance (Figure 1D) [2].

In transmission electron microscopic (TEM) cross-sections, nuclei in the meiotic zone in fli-1(ky535), including misplaced nuclei, were of roughly the same size and shape as those in wild-type (Figure 3; 2.9 ± 0.06 μm diameter for fli-1(ky535) and 3.0 ± 0.6 μm diameter for wild-type). The misplaced nuclei in fli-1(ky535) had a meiotic appearance (Figure 3). Meiotic nuclei appear round and regular as those seen in Figure 3C and 3D, whereas mitotic nuclear membranes have an irregular, "wavy" appearance. These lines of evidence indicate that the transition from mitosis to meiosis is unaffected in fli-1(ky535) mutant germ nuclei.

thumbnailFigure 3. Misplaced nuclei in fli-1(ky535) are partially surrounded by germ line plasma membrane. Shown are transmission electron microscopy cross-sections of gonads from wild-type and fli-1(ky535). (A) A cross-section through the meiotic pachytene zone of wild-type. The nuclei are arranged at the cortex (the arrowhead indicates a nucleus) and are partially surrounded by germ line plasma membrane, forming the "T" structure (arrow). (B) Cross section through the meiotic pachytene zone of a fli-1(ky535) mutant. Misplaced nuclei are apparent (arrow), and misplaced nuclei are surrounded by germ line plasma membrane in a manner similar to nuclei at the cortex (internal "T" structure-like membrane organization is indicated by the arrow). (C and D) Magnification of regions in the dashed boxes in (A) and (B). The arrowheads indicate nuclei and the arrows indicate germ line plasma membrane. Scale bars = 2 μm for all micrographs.

The previously-published fli-1(bp130) allele caused defects in oocyte production and brood size [21]. Brood size of fli-1(ky535) was comparable to that of wild-type (an average of 272 progeny for fli-1(ky535) compared to 319 for wild type; t-test p = 0.11). Possibly, bp130 is a stronger allele of fli-1 than is ky535 and affects oocyte production more strongly than ky535.

Germ line plasma membrane partially surrounded misplaced germ nuclei in fli-1(ky535)

TEM analysis revealed that wild-type meiotic zone nuclei were near the cortex. The germ line plasma membrane protruded between and partially enveloped each nucleus, forming a characteristic "T" shaped membrane described above and elsewhere (Figure 3A and 3C) [4,5]. In TEM cross-sections of meiotic regions of fli-1(ky535), germ line plasma membrane was clearly associated with each misplaced nucleus in the rachis, suggesting that germ line plasma membrane invaginated to partially enclose misplaced germ nuclei (Figure 3B and 3D and Figure 4). A similar phenotype was observed in cross-sections of animals heterozygous for a deletion of the fli-1 locus called tm362 (data not shown). No defects in the organization of the distal mitotic zone were observed in cross sections of fli-1(ky535) (e.g. the germ cell arrangement resembled wild-type and distal tip cell filopodia between germ cells was observed). While the shape and diameter of wild-type distal meiotic gonads was relatively uniform (a diameter range of 16–23 μm, n = 10), fli-1 gonads were often of irregular diameter (a range of 12–33 μm, n = 10) and irregular shape (compare Figures 3A with Figure 3B and 4A).

thumbnailFigure 4. Membrane-like structures are present between misplaced germ nuclei in fli-1(ky535). Shown are transmission electron microscope cross-sections in the meiotic pachytene zone of fli-1(ky535) mutants. (A) fli-1(ky535) displays misplaced nuclei and associated germ line plasma membrane. (B and C) Higher-magnification views of regions between the germ line plasma membrane (arrowheads) surrounding misplaced nuclei. Arrows indicate membrane-like structures in the interstices between germ line plasma membrane. An electron-dense laminar structure is shown in (C).

Sheath cell extensions were associated with misplaced nuclei in the rachis in fli-1(ky535)

The plasma membrane surrounding interior nuclei in fli-1(ky535) formed gaps between nuclei similar to the gaps formed by plasma membrane invagination around cortical nuclei (Figure 3B and 3D and Figure 4). In fli-1(ky535) mutants, additional membranes were frequently observed occupying these interior gaps formed by invaginated germ line plasma membrane (Figure 4B and 4C). Less frequently, electron-dense laminar structures were present in the interior gaps (Figure 4C). Cross sections of heterozygous fli-1(tm362)/+ deletion animals showed a similar phenotype (data not shown).

The nature of these membrane-like structures between misplaced germ cells observed by TEM was unclear. The germ line is partially surrounded by the somatic sheath cells, which extend filopodia across the bare regions of the germline not covered by the cell body [4]. Sheath cell protrusions occupy gaps between nuclei formed by germ line plasma membrane invagination. In wild-type, sheath cell protrusions do not extend deeply between nuclei but rather stay near the cortex [4]. Possibly, the membrane-like structures between misplaced germ nuclei in fli-1 mutants were somatic sheath cell extensions.

To assay sheath cell morphology, a transgene consisting of the lim-7 promoter driving gfp expression was analyzed. lim-7::gfp is expressed in the sheath cells but not the germ line [4]. In wild-type harboring lim-7::gfp, no GFP fluorescence was detected in the rachis of the meiotic region (Figure 5A, B, and 5G), although GFP was detected at the surface of the germ line in a "honeycomb" pattern as previously described [4], due to the thin cytoplasm of regions of the sheath cells covering the germ nuclei.

thumbnailFigure 5. Sheath cells projections extend into the rachis in fli-1 and let-60. (A-F) DIC and lim-7::gfp expression in wild-type, fli-1(ky535), and let-60(n2021) (the same animals at the same focal planes are shown). (A), (B) No lim-7::gfp is observed in the rachis of wild-type (arrows). (C-F) Arrows point to displaced nuclei (in the DIC images) and associated fingers of lim-7::gfp expression in the rachis of these mutants. (G-I) Merged confocal micrographs of lim-7::gfp expression (green) and DAPI nuclear staining (blue) in dissected gonads. In lfi-1(ky535) and let-60(n2021), extensions of lim-7::gfp are observed associated with displaced nuclei in the rachis (arrows). The scale bar in (A) applies to (A-F); the scale bar in (G) applies to (G-I).

In fli-1(ky535) mutants, the cortical "honeycomb" pattern was observed, although it was often irregular and disorganized, suggesting cortical nucleus arrangement was disorganized. Fingers of GFP expression were observed protruding into the rachis and associating with misplaced germ nuclei (Figure 5C, D, and 5H). These protrusions were from the somatic sheath cells and not the distal tip cell, as a lag-2::gfp transgene, expressed only in the distal tip cell [9], did not show these patches in the rachis in fli-1(ky535) animals (data not shown). The TEM and lim-7::gfp results combined indicate that misplaced nuclei in the rachis were bounded by germ line plasma membrane, and extensions of the sheath cells protruded between the misplaced germ cells in the rachis (Figure 6 is a depiction of these results).

thumbnailFigure 6. A diagram of gonad organization in fli-1. Schematic diagram of the organization of the pachytene meiotic region of the germ cell in wild-type (A) and fli-1 mutants (B). In wild-type, germ cell plasma membrane protrudes between cortical nuclei and partially envelops the, forming the "T" structure. Sheath cells protrude superficially between nuclei. In fli-1(ky535), nuclei in the rachis are enveloped with germ cell plasma membrane, and sheath cells protrude deeply into the rachis.

FLI-1 encodes a molecule similar to Flightless 1/Fliih

The ky535 mutation was mapped genetically to linkage group III by standard linkage analysis with visible markers using synthetic lethality with unc-115 (data not shown). Three-factor mapping with dpy-17 and unc-32 using the Glm phenotype indicated that ky535 was close to and to the left of unc-32 (approximately 0.22 cM) (Figure 7A). The fli-1 gene (B0523.5 on Wormbase), which encodes an actin-binding protein of the Flightless 1/Fliih family, resides in this region of the genome (Figure 7B and 7C). The FLI-1 polypeptide is composed of N-terminal leucine-rich repeats (LRRs) and 5 C-terminal gelsolin-like domains (Figure 7D). A fli-1 cDNA was isolated in a previous study [26] (U01183 in Genbank). This transcript was used as the basis for Figure 7C and 7D. The cDNA is likely to contain the entire fli-1 coding region, as an in-frame stop codon is present 5 codons upstream of the presumed initiation methionine (data not shown). Furthermore, two independent fli-1 cDNAs were sequenced (yk48g9 and yk294b7, courtesy of Y. Kohara). While incomplete at the 5' ends, these cDNAs were identical in structure to the U01183 cDNA.

thumbnailFigure 7. The fli-1 locus. (A) A genetic map of the fli-1 region. Numbers below the line indicate the number of recombination events between the loci in three-factor crosses. From dpy-17(e1264) unc-32(e189)/fli-1(ky535) trans-heterozygotes, 30/34 Dpy-non-Unc recombinants harbored ky535 and 10/99 Unc-non-Dpy recombinants harbored ky535. The estimated genetic distance between unc-32 and ky535 is 0.22 map units. (B) A diagram of the cosmid B0523. Genes on the cosmid are indicated as arrows. (C) The fli-1 gene. 5' is to the left. Black boxes indicate coding exons, and white boxes represent non-coding exons. The extent of the tm362 deletion is indicated below the line, as is the location of a Trp tRNA gene. The white box above the line indicates the region used in fli-1 RNAi experiments, and the black line represents 1 kb. The gene structure is derived from a fli-1 cDNA previously described (Genbank accession number U01183). (D) A diagram of the predicted FLI-1 polypeptide. The locations of the leucine-rich repeats (LRRs) and the five gelsolin-like repeats (G1–G5) are indicated. The deletion fli-1(tm362) removes coding region for the residues of FLI-1 indicated below the diagram. (E) A fli-1(tm362) homozygous embryo arrested before embryonic elongation. (F) A fli-1(tm362) homozygous mutant embryo arrested at the two-fold stage and showed a paralyzed arrest at two-fold (Pat) phenotype. The scale bar in (E) applies to (E) and (F).

To test if the fli-1 gene is involved in germ line morphogenesis, RNA-mediated gene interference (RNAi) of fli-1 was performed. fli-1(RNAi) phenocopied the germ line morphogenesis defect of ky535 (Figure 1F and Figure 2). Furthermore, the cosmid B0523, which contains the fli-1 gene, rescued the synthetic lethality of unc-115(mn481); fli-1(ky535) animals harboring a transgene containing the cosmid (Figure 7B). The B0523 cosmid contains two other genes, B0523.1 and B0523.3. RNAi of these genes did not cause a Glm phenocopy (data not shown). fli-1 RNAi in both wild-type and rrf-1(pk1417 and ok289) backgrounds caused the Glm phenocopy (Figure 1F and Figure 2). rrf-1 mutations attenuate RNAi in somatic cells but do not apparently affect RNAi in the germ line [27].

A PCR-generated fragment of B0523 containing only the fli-1 gene and a tryptophan tRNA (Figure 7C, see Methods) rescued the synthetic lethality of unc-115(mn481); fli-1(ky535) mutants (Figure 7C). Furthermore, a fli-1::gfp full-length fusion transgene (see Methods) partially rescued the Glm phenotype of fli-1(ky535) animals (Figure 2) as well as the lethality of unc-115(mn481); fli-1(ky535) double mutants. The nucleotide sequence of the entire region included in the rescuing fli-1(+) transgene was determined from ky535 mutants. No nucleotide changes were detected in this region in three independent PCR amplifications of the fli-1 gene from ky535 genomic DNA. Possibly, ky535 is regulatory mutation outside of the region necessary for rescue, and transgenic fli-1(+) expression, which can often lead to overexpression, can overcome the ky535 mutation. A fli-1 transcript was detected by RT-PCR in fli-1(ky535) mutants (data not shown). As described below, the fli-1 locus is haploinsufficient for the Glm phenotype, indicating that lowering fli-1 gene dosage by as little as one-half can cause the Glm phenotype.

The fli-1(tm362) deletion causes a germ line morphogenesis defect

To confirm that fli-1 controls germ line morphogenesis, a deletion in the fli-1 locus was analyzed (isolated and kindly provided by The National Bioresource Project for the Experimental Animal C. elegans, S. Mitani). The deletion, tm362, removed bases 10973 to 11931 relative to the cosmid B0523 (Genbank Accession number L07143) with breakpoints in coding exons 9 and 11 of fli-1 (Figure 7C). The out-of-frame tm362 deletion removed coding region encompassing parts of gelsolin domains 3 and 4 (Figure 7D).

fli-1(tm362) homozygotes from a heterozygous mother arrested during embryogenesis and failed to hatch. Of arrested embryos, 70% displayed the Pat phenotype (

    p
aralyzed and
    a
rrested at the
    t
wo-fold stage of embryonic elongation) (Figure 7F). The Pat phenotype is characteristic of defects in body wall muscle function [28]. Indeed, fli-1(ky535) mutants displayed slightly disorganized myofilament lattice structure in body wall muscles (data not shown), suggesting that body wall muscle development was also affected by fli-1(ky535). The remaining 30% of tm362 homozygous embryos arrested earlier in embryogenesis with severe defects in embryonic organization (Figure 7E). Defects in muscle organization and embryonic development in a fli-1 mutation have been described [21].

While homozygous fli-1(tm362) animals arrested in embryogenesis, heterozygous tm362/+ animals displayed the Glm phenotype similar to ky535 animals (49%; Figure 1E and Figure 2). TEM cross sections of tm362/+ heterozygotes were analyzed and found to have a similar ultrastructural defect as described for fli-1(ky535) (data not shown), including germ line plasma membrane and sheath cell invaginations around misplaced nuclei. These results suggest that fli-1 is haploinsufficient for the Glm phenotype. Indeed, heterozygous ky535/+ animals also displayed the Glm phenotype (60% compared to 94% for ky535 homozygotes; Figure 2). Trans-heterozygous ky535/tm362 animals were viable and had a severe Glm phenotype (91%; Figure 2), suggesting that ky535 and tm362 failed to complement for this phenotype. However, the additive effect of each heterozygote alone could explain this effect.

The lethality of fli-1(tm362) was rescued by the fli-1(+) transgene that also rescued the unc-115(mn481); fli-1(ky535) lethality (Figure 7C), and the Glm phenotype of rescued homozygous fli-1(tm362) animals was significantly less severe than fli-1(ky535) homozygotes (Figure 2). The Glm phenotype was likely due to fli-1 loss of function, as fli-1 RNAi caused the Glm phenocopy and the Glm phenotype of both fli-1(ky535) and fli-1(tm362) was rescued by transgenic fli-1(+). Thus, the viable fli-1(ky535) allele might be hypomorphic and the lethal fli-1(tm362) allele might be null. If this is the case, fli-1 might be haploinsufficient for the Glm phenotype as heterozygotes displayed the Glm phenotype. It is also possible that either or both of the two fli-1 alleles are not simple loss-of-function alleles and thus cause a dominant Glm phenotype. Indeed, fli-1(tm362) was rescued more efficiently than ky535 by transgenic fli-1(+) (Figure 2), suggesting that ky535 might have some dominant character that is more difficult to rescue. In either case, the Glm defect is likely a loss-of-function phenotype of fli-1 as RNAi of fli-1 caused the Glm defect.

Germ line actin organization in fli-1(ky535) mutants

FLI-1 can bind to and sever actin filaments [20], suggesting that it might modulate cytoskeletal organization. The effect of fli-1(ky535) on the actin cytoskeleton of the germ line was analyzed by staining with rhodamine-labeled phalloidin. Hermaphrodite somatic sheath cells contain much actin, which was difficult to distinguish from germ line actin. To circumvent this problem, male gonads, which lack sheath cells, were analyzed, although hermaphrodites showed a pattern consistent with that seen in males (data not shown).

fli-1(ky535) males displayed a Glm phenotype similar to hermaphrodites, as displaced nuclei were observed in the rachis of the single male gonad arm (Figure 8A and 8B). In the wild type male germ line, a cortical layer of phalloidin staining was associated with the germ line plasma membrane that surrounded each germ nucleus (Figure 8C). In fli-1(ky535), nuclei at the cortex displayed an apparently normal actin organization. However, a cortical layer of actin was observed surrounding the misplaced nuclei in the rachis, apparently associated with the invaginated plasma membrane (Figure 8D). While actin was associated with misplaced nuclei in fli-1(ky535), no obvious defects in the organization of the actin cytoskeleton per se were observed. The fli-1(bp130) allele caused defects in gonad actin organization [21] not seen in fli-1(ky535). fli-1(ky535) might be a hypomorphic allele, and actin organization might not be affected to the extent observed in bp130.

thumbnailFigure 8. Actin organization in the fli-1(ky535) male germ line. (A) and (B). DIC images of the wild-type and fli-1(ky535) male germ lines in the meiotic zone. The arrow in (A) points to the rachis in wild-type, devoid of germ nuclei. The arrowheads in (B) point to misplaced nuclei in the rachis of the meiotic zone in fli-1(ky535). (C) and (D) Rhodamine-phalloidin staining of male gonads. (C) Cortical actin surrounds each germ nucleus, and the arrowhead indicates increased intensity at presumptive "T" structures. The rachis is narrower in the male germ, but the rows of cortical nuclei can be clearly observed in this micrograph. (D) In fli-1(ky535), cortical actin surrounds misplaced nuclei in the rachis, but actin organization per se is not obviously affected. Scale bars represent 10 μm.

fli-1 is expressed in the gonad and in muscle

A transcriptional fli-1promoter::gfp reporter transgene was constructed that contained the fli-1 5' upstream region driving gfp (see Methods). Expression was observed in body wall muscle, pharyngeal muscle, and vulval muscle of embryonic, larval, and adult animals (Figure 9A). This is consistent with the Pat phenotype of fli-1(tm362) animals and the pharyngeal pumping defects of unc-115; fli-1(ky535) animals. In complex arrays (see Methods), expression was occasionally observed along the entire length the adult gonad in a "honeycomb" pattern characteristic of gonad expression (Figure 9B). This expression was faint and variable (not observed in all animals) and tended to dissipate as the complex array lines were maintained for more than three generations. This pattern could reflect expression in the germ line, the somatic sheath cells, or both. Male gonads, which are devoid of sheath cells, also showed faint and variable expression along their lengths (Figure 9B inset), suggesting that expression might be in the germ line. However, sheath cell expression cannot be excluded from these experiments.

thumbnailFigure 9. fli-1::gfp is expressed in the gonad and in muscle. Shown are epifluorescence images of wild-type animals harboring a transgene consisting of the fli-1 promoter driving gfp expression. (A) Arrows point to pfli-1::gfp expression in body wall muscle, pharyngeal muscle, and vulval muscle (inset). (B) Arrows point to germ line expression in a hermaphrodite and a male (inset). The "honeycomb" pattern is due to exclusion of GFP from the nuclei of the germ line.

The full-length fli-1::gfp transgene, predicted to encode a full-length FLI-1 polypeptide with GFP at the C-terminus, rescued fli-1 lethality and partially rescued the Glm phenotype of fli-1(ky535) and fli-1(tm362), suggesting the FLI-1::GFP molecule was functional. No FLI-1::GFP fluorescence was detected in the gonads of these transgenic animals, and muscle expression was very faint and inconsistent. Possibly, FLI-1::GFP was expressed at very low levels, below detection in the gonad.

To detect low levels of FLI-1::GFP expression, gonads from animals expressing full-length fli-1::gfp were excised and stained with an antibody against GFP. Specific GFP immunoreactivity was predominantly associated with germ nuclei (Figure 10A–F). Gonads from animals without the fli-1::gfp transgene showed no such reactivity (Figure 10G–I). FLI-1::GFP was associated with nuclei along the length of the entire gonad, and no obvious differences in FLI-1::GFP accumulation or nuclear association were detected along the length of the distal gonad from the mitotic zone through the meiotic zone.

thumbnailFigure 10. FLI-1::GFP accumulates at germ line nuclei. Confocal images of gonads from animals stained with anti-GFP antibody (green) and DAPI to label nuclei (blue). (A-F) are images of gonads from wild-type animals harboring the full-length fli-1::gfp transgene that rescues the fli-1 Glm phenotype and that is predicted to encode a full-length FLI-1 protein tagged with GFP at the C-terminus. (G-I) are images of a wild-type animal without the fli-1::gfp transgene. (A-C) are images of the transition zone (upper right) to pachytene zone (lower left); (D-F) are images of the pachytene zone. FLI-1::GFP reactivity was found associated with germ line nuclei. The scale bars in A and G represent 5 μm for A-C and G-I. The scale bar in D represents 2 μm for D-F.

let-60 mutations display a germ line morphogenesis phenotype similar to fli-1

Previous studies described defects in germ line organization in mutants of Ras signaling pathway components: mpk-1 and ksr-2 mutations caused germ line clumping [17,29]; and mek-2 and let-60 Ras mutants displayed misplaced nuclei in the meiotic zone [16]. C. elegans LET-60 is similar to human k-Ras [30,31], and has been shown to control transition of germ nuclei from meiotic pachytene to diakinesis and germ line organization [16].

To begin to characterize Ras signaling in the Glm phenotype, alleles of let-60 Ras that cause loss of function, constitutive activation, and dominant negative effects were analyzed for the germ line morphogenesis defect by DIC optics and DAPI staining (Figure 11A) [30,32,33]. The hypomorphic loss-of-function allele n2021 caused a ky535-like germ line defect in 44% of gonad arms, and the stronger let-60 loss-of-function alleles s1124, s1155, and s59, which are homozygous lethal, caused the Glm phenotype when heterozygous (52%, 23%, and 47%, respectively). These data suggest that let-60 is haploinsufficient for the Glm phenotype as was fli-1. Three different dominant-negative alleles of let-60 also displayed the germ line phenotype as homozygotes or as heterozygotes (e.g. 94% for homozygous sy93) (Figure 11A). TEM sections of let-60(n2021) showed a similar ultrastructural defect as fli-1(ky535) (data not shown), including germ line plasma membrane and sheath cell protrusions between misplaced nuclei. Furthermore, let-60 loss-of-function and dominant negative mutants displayed sheath cell lim-7::gfp expression associated with misplaced nuclei (Figure 5E, F, and 5I). While loss-of-function and dominant negative let-60 alleles caused the Glm phenotype, constitutively-active let-60 alleles n1700 and n1046 caused little or no Glm phenotype (Figure 11A).

thumbnailFigure 11. Constitutively-active LET-60 suppresses the fli-1 Glm phenotype. Genotypes are along the x axis, and percentage of gonad arms displaying the Glm phenotype are along the y-axis. Numbers of gonads scored for each genotype are indicated. Error bars represent the standard error of the proportion, and matching numbers of asterisks indicate that the genotypes are significantly different (t-test and Fisher's Exact analysis; p < 0.001). (A) Loss-of-function and dominant-negative alleles of let-60 Ras displayed the Glm phenotype whereas constitutively-active alleles did not. (B) Two constitutively-active alleles of let-60 Ras suppress the Glm phenotype of fli-1(ky535) and fli-1(tm362)/+.

LET-60 activity can compensate for loss of FLI-1 in germ line morphogenesis

fli-1 and let-60 Ras mutations cause the Glm defect, and constitutively-active let-60 alleles, which presumably cause let-60 overactivation, had no apparent effect on germ line morphogenesis. The constitutively-active let-60(n1700) mutation partially suppressed the Glm defect of fli-1(ky535) heterozygotes and homozygotes and fli-1(tm362)/+ heterozygotes (Figure 11B). For example, fli-1(tm362) heterozygotes displayed 49% defective gonad arms, reduced to 15% by heterozygous let-60(n1700). let-60(n1046), another constitutively-active let-60 mutation, suppressed the Glm phenotype of fli-1(ky535)/+ heterozygotes (60% versus 18%). These data indicate LET-60 Ras overactivation can partially compensate for loss of fli-1 function and suggest that fli-1 and let-60 Ras act together to control germ line morphogenesis. Possibly, FLI-1 and LET-60 act in the same pathway or in parallel pathways to control germ line morphogenesis. It is also possible that FLI-1 and LET-60 control each others' expression.

Discussion

Experiments described here show that mutations in fli-1 and let-60 Ras affect morphogenesis of the germ line (the Glm phenotype). fli-1 can encode an actin-binding protein similar to Drosophila and human Flightless-1 and has been shown to interact physically with human Ha-Ras via the leucine-rich repeats [20]. Studies described here show that overactivity of let-60 Ras can compensate for fli-1 loss-of-function in the germ line, suggesting that FLI-1 and LET-60 Ras act together to control germ line morphogenesis.

The Glm phenotype

Meiotic nuclei are associated with the cortex of the germ line, forming the rachis. Sheath cell filopodia protrude superficially in the gaps between germ line plasma membrane that partially surround meiotic nuclei, but they do not protrude deeply [4]. fli-1 mutants displayed interconnected chains of meiotic nuclei spanning the rachis of the meiotic zone, a configuration never seen in wild-type. These misplaced nuclei were partially surrounded by invaginations of germ line plasma membrane as were their normally-positioned counterparts at the cortex. Gonadal sheath cell projections protruded between the plasma membrane invaginations and were in close proximity to the nuclei deep in the center of the rachis region (see Figure 6 for a diagram of these results). Such deep sheath cell projections in the meiotic zone were never observed in wild type. In fli-1 mutants, the projections between misplaced nuclei in the meiotic zone were not from the distal tip cell, but were from the more proximally-located sheath cells that normally do not protrude deeply between nuclei.

Misplaced chains of nuclei in fli-1 mutants were not due to defects in meiotic progression, as all aspects of meiosis appeared normal in fli-1 mutants: nuclei in the meiotic zone did not incorporate BrdU, suggesting that they were post-mitotic; the morphology of misplaced nuclei was similar to normal meiotic nuclei as judged by DAPI staining and by electron microscopy. Thus, while fli-1 mutant germ nuclei apparently underwent meiosis normally, they failed to organize properly to form the rachis.

Data presented here suggest that fli-1 affects germ line morphogenesis without affecting meiotic progression or other aspects of germ line differentiation. However, fli-1(ky535) is a hypomorph and fli-1(tm362) homozygotes arrested in embryogenesis before germ line development (the germ line phenotype was scored in fli-1(tm362) heterozygotes). All fli-1 genotypes in which the Glm phenotype was scored had some fli-1 activity, so it is possible that complete loss of fli-1 activity in the germ line would affect other aspects germ line development not apparent in these studies (e.g. meiotic progression to diakinesis similar to let-60 Ras or other aspects of meiotic differentiation). Possibly, FLI-1 is required for the proper formation or maintenance of the rachis through effects on the actin cytoskeleton or the germline plasma membrane. Alternatively, FLI-1 might be part of a developmental program that coordinates rachis formation with other aspects of meiotic differentiation.

The Glm phenotype might be sensitive to gene dosage

Animals heterozygous for both fli-1(ky535) and fli-1(tm362) displayed the Glm phenotype. let-60 Ras was also haploinsufficient, as heterozygous let-60 Ras loss-of-function mutations displayed the Glm phenotype. These data suggest that precise dosages of FLI-1 and LET-60 Ras are required for normal germ line morphogenesis, and that reduction by as little as one half can cause the Glm phenotype. It is also possible that ky535 and tm362 are not simple loss-of-function alleles and that each might have a gain-of-function effect, explaining the Glm defect of heterozygous animals. In any case, RNAi of fli-1 results in the Glm phenotype, suggesting that the Glm phenotype is a consequence of loss of fli-1 function.

No nucleotide lesion associated with ky535 was detected in the region that can rescue the fli-1(ky535) and fli-1(tm362) Glm phenotypes. However, ky535 was mapped genetically to the fli-1 region using the Glm phenotype, and fli-1 RNAi phenocopied the ky535 phenotype. Furthermore, the Glm phenotype of ky535 was rescued by a fli-1(+) transgene containing only the fli-1 gene. Possibly, ky535 is a mutation outside of the rescuing region that reduces but does not abolish fli-1 expression, such as a mutation in a distal enhancer element. The haploinsufficiency of the fli-1 locus is consistent with this idea. The fli-1(tm362) deletion also displayed a haploinsufficient Glm phenotype rescued by a fli-1(+) transgene.

FLI-1 and LET-60 Ras might act together to control germ line morphogenesis

let-60 Ras loss-of-function and dominant-negative mutations caused the Glm phenotype similar to fli-1. Constitutively-active alleles of let-60 Ras did not. Previous studies showed a germ cell organization defect in let-60 and mpk-1 mutants [16,17]. mpk-1 caused large clumps of nuclei with regions of the germline barren of nuclei, a defect rarely seen in the fli-1 and let-60 analyses described here. Possibly, the defects of fli-1 and mpk-1 are related, and mpk-1 has a stronger effect than fli-1. Alternatively, fli-1 and mpk-1 might affect distinct processes.

Interestingly, the Glm phenotype of fli-1 mutations was suppressed by constitutively-active let-60 Ras mutations, suggesting that LET-60 Ras overactivity compensated for FLI-1 loss of function. In these experiments, FLI-1 activity was reduced but not eliminated (ky535 is a hypomorph, and tm362 was heterozygous). Thus, it is possible that LET-60 Ras and FLI-1 act together in the same pathway or in parallel pathways to control germ line morphogenesis. The LRRs of C. elegans FLI-1 interact physically with human Ha-Ras in vitro [20] suggesting that FLI-1 and Ras might act in the same pathway. Another possibility is that let-60 controls fli-1 expression. Indeed, microarray expression analysis indicates that fli-1 transcript levels are increased by constitutively-active let-60(G12V) [34]. Further experiments will be required to test these models of FLI-1 and LET-60 interaction.

FLI-1 is expressed in the gonad

The fli-1 promoter was active in muscle cells and in the gonad. Anti-GFP Immunofluorescence revealed that FLI-1::GFP was associated with germ line nuclei. The expression pattern of fli-1 is consistent with expression in the germ line, but expression in the somatic sheath cells cannot be excluded. Furthermore, rescue of the fli-1(ky535) and fli-1(tm362) Glm phenotype could be due to somatic or germline transgene expression. FLI-1 might be expressed and active in the germ line, in the somatic sheath cells, or both. RNAi of fli-1 in rrf-1 mutants led to the Glm phenocopy, suggesting that knock-down of fli-1 in the germ line causes the Glm phenotype. However, fli-1 is very sensitive to gene dosage, so even slight perturbation of fli-1 in the soma of rrf-1 animals might be enough to cause the phenotype. fli-1 males also showed the Glm phenotype, and male gonads do not have somatic sheath cells. Together, these data suggest that fli-1 acts in the germ line, but they do not exclude the possibility that fli-1 acts in the sheath cells or in another tissue.

Human Fliih acts in the nucleus as a component of a coactivator complex and with the TCF/LEF and β-catenin complex [22,23]. However, Fliih also associates with microtubule- and actin-based structures in the cytoplasm of fibroblasts, and acts with small GTPase and PI3 kinase signaling in the cytoplasm [35]. It is unclear from these experiments if FLI-1 acts in the nucleus or cytoplasm in germ line morphogenesis. FLI-1 could act in the nucleus to regulate expression along with Ras signaling. Alternatively, FLI-1 could act in the cytoplasm in a pathway parallel to a transcriptionally-dependent Ras pathway, possibly by modulating cytoskeletal architecture involved in germ line reorganization, although no defects in germ line actin organization were apparent. Further studies will address these models of molecular mechanisms of FLI-1 and LET-60 Ras function in germ line morphogenesis.

Conclusion

This work describes the role of the FLI-1 molecule in germ line morphogenesis in C. elegans. While much is known about meiotic differentiation in C. elegans, less is known about the mechanisms that control meiotic germ cells organization at the periphery of the germ line to form a germ cell-free core of cytoplasm called the rachis. Mutations in fli-1 perturb rachis organization without perturbing meiotic differentiation. In fli-1, germ cell nuclei occupied positions in the rachis; these misplaced nuclei were partially enclosed by germ line plasma membrane as were nuclei at the cortex; and extension of the gonadal sheath cells were associated with misplaced nuclei deep in the rachis. Mutations in let-60 Ras also displayed this phenotype, and constitutively-active LET-60 partially compensated for loss of FLI-1, indicating that LET-60 Ras and FLI-1 might act together to control germ line morphogenesis. These studies describe a developmental role for the FLI-1 molecule in germ line morphogenesis and demonstrate a functional interaction between FLI-1 and Ras GTPases in this process.

Methods

C. elegans strains and genetics

C. elegans were cultured by standard techniques [36,37]. All experiments were done at 20°C unless otherwise noted. The Bristol strain N2 was used as the wild-type. The following mutations and transgenes were used. LGX: unc-115(mn481), sem-5(n2089). LGI: mek-2(n1989), sur-2(ku9). LGII: let-23(n1045), let-23(sy10), lin-31(n301). LGIII: fli-1(ky535), fli-1(tm362), tnIs6 [plim-7::gfp], dpy-17(e164), unc-32(e189), mpk-1(ku1), eT1. LGIV: let-60(n2021), let-60(s1124), let-60(s1155), let-60(s59), let-60(sy93), let-60(sy92), let-60(sy99), let-60(n1046), let-60(n1700), lin-3(e1417), lin-3(n1058), lin-1(n431). LGV: sos-1(s1031), lin-25(e1446), qIs56 [lag-2::gfp].

Transgenic C. elegans were produced by germ line microinjection of DNA solutions using standard techniques [38]. Cosmid DNAs were injected at 100 ng/μl, and fli-1 fragments generated by PCR were injected at 25 ng/μl. To visualize germ line expression of fli-1 transgenes, complex arrays were constructed using fragmented C. elegans genomic DNA in the injection mix [39]. fli-1::gfp expression in the germ line was unstable and became non-visible as the transgenes were propagated. For the fli-1::gfp immunofluorescence experiments, new complex-array transgenic lines were produced before each experiment to ensure robust fli-1::gfp expression in the germ line.

The germ line morphogenesis phenotype (Glm) was quantitated by scoring the percentage of gonad arms that displayed chains of nuclei spanning the rachis of the meiotic pachytene zone. In wild-type, chains of nuclei were often observed in the transition zone where reorganization occurs. Care was taken to ensure that the Glm phenotype was scored clearly in the meiotic pachytene zone and not in the transition zone. Significance of quantitative data was determined by the t-test and by Fisher's Exact analysis (for percentages).

fli-1 molecular biology

Fragments of the fli-1 gene were amplified using polymerase chain reaction (PCR). The sequence of all coding regions generated by PCR were determined to ensure that no errors were introduced. The fli-1 whole gene consisted of bases 8,674,953–8,684,714 of linkage group III. For fli-1(ky535) sequencing, this region was amplified in three overlapping fragments and their sequences determined. In three separate amplifications, no nucleotide changes were detected in fli-1(ky535) DNA. The full-length fli-1::gfp transgene was produced by amplifying a region including the fli-1 upstream and fli-1 coding region but not including the stop codon or downstream region (bases 8,676,004–8,684,714 linkage group III). This fragment was then fused in-frame to gfp in vector pPD95.77 (kindly provided by A. Fire). The fli-1 promoter::gfp fusion was produced by amplifying the fli-1 upstream region (8,683,600–8,684,714 of linkage group III) and fusing the fragment upstream of gfp in pPD95.77. RNA-mediated gene interference (RNAi) was performed by microinjection of double-stranded RNA [40], representing a portion of fli-1 exon 6 (see Figure 4), into the germ line and analyzing the germ line phenotype in progeny of injected animals. The sequences of all oligonucleotide primers used in this study are available upon request.

Imaging and microscopy

Differential Interference Contrast (DIC) and epifluorescence images were taken using a Leica DMR light microscope with a Hamamatsu Orca camera. Some images were obtained with an Olympus spinning disk confocal microscope. For electron microscopy, samples were examined and photographed using a JEOL1200EXII transmission electron microscope and a MegaView camera (Soft Imaging System). Images were adjusted for contrast, cropped, and overlayed using Adobe Photoshop.

TEM specimen preparation and analysis

Hermaphrodites (12 hours after the L4/adult molt) were fixed using a modification of the procedure previously described [41]. Worms were anaesthetized immersing them in 8% EtOH in M9 buffer for 5 minutes and were fixed by immersion in 2.5% glutaraldehyde, 1% formaldehyde in 0.1 M sucrose, 0.05 M Na-cacodylate, pH7.4, for 30 min at 4°C. Animals then were cut in half using a scalpel and returned to the fixative and incubated overnight at 4°C. They then were rinsed 3 times (10 minutes each) at 4°C with 0.2 M Na-cacodylate, pH 7.4, and then post-fixed for 90 minutes, at 4°C, with 0.5% OsO4, 0.5% KFe(CN)6 and 0.1 M Na-cacodylate, pH 7.4, for 90 minutes on ice. Subsequent steps were carried out at room temperature. Worms then were rinsed three times, 10 minutes each, in 0.1 M Na-cacodylate buffer, stained in 1% uranyl acetate in 0.1 M sodium acetate, pH 5.2, for 1 hour at room temperature, followed by three 5-minute 0.1 M sodium acetate washes and three 5-minute distilled water washes. Worms were packed in parallel in a V-shaped plexiglass trough and were embedded 3% seaplaque agarose. Approximately 1 mm2 blocks then were dehydrated in acetone and embedded in Embed 812 [42].

For each genotype examined, at least three individual animals were sectioned, and multiple sections from each animal along the entire gonad span were analyzed. Cross-sections of worms were cut using a diamond knife and Leica microtome and were picked up on carbon-over-formvar coated single hole grids. Sections were dried overnight and then stained using minor modifications of the Hall (1995) procedure. Stains and washes were prepared in 16 well plastic culture dishes at room temperature. Grids were stained in 1% uranyl acetate, 50% methanol for 15 minutes, rinsed twice (30 seconds each) with 100% ethanol followed by 50% ethanol/water (15 seconds), 30% ethanol/water (15 seconds), and four 15 second washes in water. Sections then were stained for 5 minutes with 0.1% lead citrate in 0.1 M NaOH, rinsed twice with 0.02 M NaOH (1 minute/change), rinsed five times in water (15 seconds/wash) and were air-dried before examination with the TEM.

DAPI and phalloidin staining of dissected gonads

The gonads of 12-hour-old adult hermaphrodite animals were dissected and fixed with 3% paraformaldehyde containing 0.1 M K2HPO4, pH7.2, for 1 hour at room temperature. The specimens were washed once with phosphate-buffered saline (PBS) with 0.1% Tween-20 (PBT) for 5 minutes followed by treatment with 100% methanol for 5 minute at -20°C. Specimens were treated with PBS containing 100 ng/μl 4',6-diamidino-2-phenylindole (DAPI) or rhodamine-phalloidin for 10 minutes at room temperature followed by three washes in PBT. Gonads were mounted on a 2% agarose pad in M9 buffer with 1 mg/ml 1,4-diazabicyclo [2.2.2]octane (DABCO) antifade reagent.

BrdU labeling of dissected gonads

Escherichia coli strain MG1693 (a thymidine-deficient E. coli strain kindly provided by the E. coli stock center) were grown minimal medium (M9) with 0.4% glucose, 1 mM MgSO4, 1.25 μg/ml vitamin B1, 0.5 μM thymidine, and 10 μM bromodeoxyuridine (BrdU) overnight at 37°C [25]. BrdU-labeled E. coli were then plated on nematode growth medium (NGM) plates containing 100 μg/ml ampicillin. 12-hour-old adult hermaphrodite animals were placed on seeded plates and allowed to eat the BrdU-labeled E. coli for varying times depending on the experiment (usually 5 minutes). Gonads were dissected immediately and fixed in methanol at -20°C for 1 hour followed by 1% paraformaldehyde for 15 minutes at room temperature.

Fixed gonads were placed in 1 mg/ml BSA in PBT for 15 minutes, 2N HCl to denature DNA for 30 minutes at room temperature, and 0.1 M sodium borate to neutralize for 15 minutes at room temperature. The specimens were blocked in 1 mg/ml BSA in PBT for 15 minutes and stained with a 1:2.5 dilution in PBT of anti-BrdU antibody (B44, Becton-Dickinson, San Jose, CA) at 4°C overnight. On the next day, the specimens were washed three times by 1 mg/ml BSA in PBT for 10 minutes each. A 1:500 dilution of Alexa 488-conjugated goat-anti-mouse antibody was incubated with the specimens at room temperature for 2 hours in PBT. The specimens were washed three times with 1 mg/ml BSA in PBT for 10 minutes each with DAPI in the last wash to stain DNA (see above). Gonads were mounted for microscopy as described above.

Anti-GFP immunofluorescence of dissected gonads

Gonads of adult hermaphrodite animals (12 hours after the L4/adult molt) were fixed as described for DAPI staining. Fixed gonads were blocked for 1 hour in 1 mg/ml BSA in PBT at room temperature and then were incubated overnight with 1:50 diluted monoclonal anti-GFP (Sigma-Aldrich, St. Louis, MO) antibody at 4°C. The specimens were washed three times with PBT for 10 minutes each and incubated with 1:500 diluted Alexa 488-conjugated goat-anti-mouse antibody (Sigma-Aldrich, St. Louis, MO) for 2 hours at room temperature. DAPI was included to stain DNA. Stained gonads were rinsed three times with PBT for 10 minutes each. Gonads were mounted for microscopy as described above.

Authors' contributions

JL conducted all of the experiments described in the manuscript and participated in figure construction and manuscript writing. WLD assisted with transmission electron microscopy and discussion of results. EAL oversaw all aspects of the project and contributed the bulk of manuscript writing.

Acknowledgements

Thanks to E. Struckhoff for technical assistance, T. Schedl for helpful discussions and suggestions, S. Mitani and the National Bioresource Project for the Experimental Animal "Nematode C. elegans" for fli-1(tm362), the Caenorhabditis Genetics Center, sponsored by the National Center for Research Resources, for providing C. elegans strains, the Sanger Center and C. elegans genome Sequencing Consortium for cosmids, and Y. Kohara for cDNA clones. This work was supported by NIH grant NS40945 and NSF grant IBN93192 to E.A.L., and NIH grant P20 RR016475 from the INBRE Program of the National Center for Research Resources.

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