Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatin

Additional file 1:

Representative cells from birth-dating experiment using [³H]-dT in combination with Prox1. High power bright-field micrograph of dissociated st35 retinal cells labelled for Prox1 (DAB colorimetric staining, brown) and processed for autoradiographical detection of [³H]-dT incorporation.

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Additional file 3:

Data presented in figures 2 and 6 to 9. Data used for making graphs in figures 2, 6, 7, 8 and 9.

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Additional file 2:

Follistatin expression in st34 retina. Follistatin immunoreactivity (red) in a st34 retina is restricted to the ganglion cell layer (gcl) and to certain cells located on the inner-most rim of the inner nuclear layer (inl). This pattern together with the observation that follistatin treatment cause a thinning of the inner plexiform layer (this study and ref [28]), and our data demonstrating that follistatin mRNA levels increase from st35 and beyond suggest that follistatin also has a function in the development, organization and/or establishment of the inner plexiform layer. Scale bar: 20 μm.

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