IGF1-induced bone growth partially requires PI3K activity. (A)Tibiae isolated from E15.5 mice were cultured for 6 days in the presence of IGF1 or 0.1% BSA in PBS, as control. IGF1 induced significant increase in bone growth. (B) Measurements of bone growth after 6 days of treatment with IGF1, control, LY294002 or IGF1 + LY294002 showed increased bone growth in the IGF1 treatment, and similar bone growth between the control and the IGF1 + LY294002 treatment. (C) E 15.5 tibiae treated for 6 days with the treatments mentioned above, were fixed, embedded, sectioned and stained with Safranin O. The length of hypertrophic zone was increased in the IGF1 treatment compared to control; decreased in the LY294002 treatment as shown before and in the case of the IGF1 + LY294002 treatment similar to the control hypertrophic zone length.
Ulici et al. BMC Developmental Biology 2008 8:40 doi:10.1186/1471-213X-8-40