Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants

doi:10.1186/1471-213X-8-31

BMC Developmental Biology

Volume 8

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Research article

Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants

Cheng-chen Huang¹ , Chin-Wei Huang¹ , Yih-Shyun E Cheng² and John Yu¹^,2

¹Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan

²Genome Research Center, Academia Sinica, Taipei, Taiwan

author email corresponding author email

BMC Developmental Biology 2008, 8:31doi:10.1186/1471-213X-8-31


Published:	25 March 2008

Additional files

Additional file 1:

TEM image of the regenerating blood vessels of wild-type fish. A representative TEM image of a wild-type 3-dpa regenerate shows vascular plexus evident by the narrow canal (black arrow) and connection (white arrow) between blood vessels that are of different sizes. B, blood cell; E, endothelial cell; BV, blood vessel. Scale bar, 2 μm.

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Additional file 2:

TEM images of the regenerating blood vessels of reg6 fish. Composite TEM images of a reg6 3-dpa regenerate show enlarged blood vessel (lower left cornor) which is filled with blood cells. Blood cells are often leaked out of the blood vessels (arrows). The endothelial cells in reg6 are less stretch-out and sometimes stacked (arrowhead). However, no clear cell junction is found among these cells. B, blood cell; E, endothelial cell. Scale bar, 2 μm.

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Additional file 3:

Expression levels of VEGF related genes in wild-type and reg6 regenerative fins. The expression levels of VEGFC, VEGFD, VEGFR1, and VEGFR2 is about the same in wild-type (black bars) and reg6 (white bars) 3-dpa regenerates. Note that the level of VEGFC seems higher but the magnitude is less than two folds. The expression level is determined by QPCR and normalized by the expression level of β-actin gene.

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Additional file 4:

Expression levels of VEGF related genes in reg6 regenerative fins upon different treatments. The treatment of (1) H₂O, (2) 1 mM histamine, (3) 10 μM SKF91488, or (4) 300 μM urocanic acid does not alter significantly the expression levels of VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, and VEGFR3 in reg6 3-dpa regenerates.

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