Generation of TAP modified fosmids with pMOD4-galK-GT. Fosmids carrying dod-22 (top) or srp-6 genes (bottom) were modified to generate a C-terminal (dod-22) and N-terminal (srp-6) TAP fusion transgene. After modification, pLoxP unc-119 was then integrated into the fosmid backbone. The effectiveness of fosmid modification was monitored via PCR using the indicated oligo sets. Lane 1 and 2 C-term TAP F and C-term TAP R (top) and N-term TAP F and N-term TAP R (bottom); Lane 3 and 4 primers derived from the sequences flanking the insertion site which produce an up-shift following insertion of the tag (sequence available upon request); Lane 5 and 6 unc-119 F and unc-119 R.
Zhang et al. BMC Developmental Biology 2008 8:119 doi:10.1186/1471-213X-8-119