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Open Access Highly Accessed Methodology article

A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering

Yue Zhang12, Lindsey Nash1 and Alfred L Fisher1*

Author Affiliations

1 University of Pittsburgh, Department of Medicine, Division of Geriatric Medicine and Pittsburgh Institute for Neurodegenerative Diseases, Pittsburgh, PA 15260, USA

2 Dept. of Radiation Oncology, BIDMC, Boston, MA 02215, USA

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BMC Developmental Biology 2008, 8:119  doi:10.1186/1471-213X-8-119

Published: 30 December 2008

Additional files

Additional file 1:

Legend for overview of recombineering procedures. Figure legend for Additional files 2 and 3.

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Additional file 2:

Merged overview of recombineering procedures. A merged figure showing the steps and time involved in recombineering using the original RT cassette, modified RT cassette, and galK cassette.

Format: PPT Size: 144KB Download file

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Additional file 3:

Separate overviews of recombineering procedures. Separate figures showing the steps and time involved in recombineering using the original RT cassette, modified RT cassette, and galK cassette. These are the same figures that are merged in Additional file 2, but provided separately for ease of reading or printing.

Format: PPT Size: 121KB Download file

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Open Data