Table 2 |
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|
Tissue restricted expression of the genes isolated by whole mount in situ hybridization. |
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|
Gene symbol |
Gene name |
L |
F |
E |
H |
T |
Expression pattern in E8.0–8.5 |
|
|
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|
Group1: Genes expressed in definitive endoderm only |
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|
|
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|
*Pyy |
peptide YY |
19 |
14 |
9 |
0 |
23 |
foregut |
|
5730521E12Rik |
5730521E12Rik |
9 |
9 |
25 |
63 |
97 |
midgut |
|
|
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|
Group2: Genes expressed in both definitive and yolk sac endoderm |
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|
|
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|
Cldn9 |
Claudin9 |
18 |
4 |
4 |
1 |
9 |
definitive endoderm and yolk sac |
|
Habp2 |
Hyaluronic binding protein 2 |
25 |
0 |
2 |
4 |
6 |
definitive endoderm and yolk sac |
|
Spp2 |
Secreted phosphoprotein2 |
16 |
1 |
7 |
2 |
10 |
definitive endoderm and yolk sac |
|
Ttr |
Transthyretin |
29 |
4 |
1 |
7 |
12 |
definitive endoderm and yolk sac |
|
|
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|
Group3: Genes expressed in definitive endoderm, yolk sac and other germ layers |
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|
|
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|
Cpn1 |
Carboxypeptidase N, polypeptide1 |
28 |
2 |
5 |
6 |
13 |
definitive endoderm, neural tube and yolk sac |
|
1700011H14Rik |
1700011H14Rik |
27 |
1 |
2 |
4 |
7 |
definitive endoderm(weak), neural tube and yolk sac |
|
Mogat2 |
Monoacylglycerol O-acyltransferase2 |
36 |
2 |
11 |
6 |
19 |
yolk sac endoderm, definitive endoderm, anterior ectoderm, and somite |
|
Spink3 |
Serine peptidase inhibitor, Kazal type3 |
38 |
44 |
109 |
57 |
210 |
yolk sac endoderm, ectoderm and definitive endoderm |
|
Phlda2 |
Pleckstrin homology-like domain, family A, member2 |
26 |
7 |
10 |
5 |
22 |
yolk sac endoderm, lateral plate mesoderm and ventral definitive endoderm |
|
Trap1a |
Tumor rejection antigen P1A |
17 |
1 |
5 |
6 |
12 |
definitive endoderm, yolk sac and midbrain and tailbud |
|
|
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|
Group4: Genes not expressed in yolk sac and heart, but expressed in other germ layers |
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|
|
|||||||
|
Gabpb1 |
GA repeat binding protein, beta1 |
27 |
3 |
1 |
2 |
6 |
entire definitive endoderm, head and tailbud ectoderm and mesoderm, but not expressed at heart and yolk sac |
|
*Cdx1 |
Caudal type homeo box1 |
13 |
0 |
17 |
4 |
21 |
3 germ layers of the posterior embryo |
|
*Trh |
Thyrotropin releasing hormone |
18 |
4 |
9 |
0 |
13 |
definitive endoderm, brain, midline |
|
*Cyp26a1 |
Cytochrome P450, family 26, subfamily a, polypeptide1 |
24 |
0 |
5 |
9 |
14 |
tailbud |
|
*Tbx1 |
T-box1 |
14 |
4 |
1 |
0 |
5 |
foregut and mesoderm |
|
*Otx2 |
Orthodenticle homolog2 |
27 |
8 |
2 |
0 |
10 |
brain, foregut |
|
Arg1 |
Arginase 1, liver |
15 |
4 |
0 |
4 |
8 |
3 germ layers of the trunk, but not expressed at head, heart, tail bud and yolk sac |
|
Gm784 |
Gene model 784 |
37 |
6 |
1 |
5 |
12 |
3 germ layers, but not expressed at heart and yolk sac |
|
A230098A12Rik |
A230098A12Rik |
10 |
3 |
1 |
1 |
5 |
3 germ layers, but not expressed at heart and yolk sac |
|
Usp22 |
Ubiquitin specific peptidase |
38 |
2 |
3 |
3 |
8 |
3 germ layers, but not expressed at heart and yolk sac |
|
|
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|
Group5: Genes expressed in yolk sac endoderm only |
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|
|
|||||||
|
Tdh |
L-threonine dehydrogenase |
19 |
1 |
7 |
11 |
19 |
yolk sac endoderm |
|
Lgals2 |
Lectin, galactose-binding, soluble 2 |
18 |
1 |
2 |
6 |
9 |
yolk sac endoderm |
|
Cubn |
Cubilin (intrisic factor-cobalamin receptor) |
23 |
1 |
16 |
17 |
23 |
yolk sac endoderm |
|
Pla2g12b |
Phospholipase A2, group12B |
16 |
2 |
2 |
1 |
5 |
yolk sac endoderm |
|
Apoc2 |
ApolipoproteinC-2 |
17 |
3 |
13 |
7 |
23 |
yolk sac endoderm |
|
|
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|
L: number of libraries which the 3' most tag sequence of each gene present in; F, E, H, T: raw counts of the 3' most tag sequences for each gene in foregut library (F), whole endoderm library (E), hindgut library (H) and in the three endoderm libraries (T) respectively. *: genes validated in the first list. |
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|
Hou et al. BMC Developmental Biology 2007 7:92 doi:10.1186/1471-213X-7-92 |
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