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Open AccessHighly AccessResearch article

A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE)

Juan Hou1 email, Anita M Charters2 email, Sam C Lee1 email, Yongjun Zhao2 email, Mona K Wu1 email, Steven JM Jones2,3 email, Marco A Marra2,3 email and Pamela A Hoodless1,3,4 email

1Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada

2Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, Canada

3Department of Medical Genetics, University of British Columbia, Vancouver, Canada

4Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada

author email corresponding author email

BMC Developmental Biology 2007, 7:92doi:10.1186/1471-213X-7-92

Published: 2 August 2007

Additional files

Additional file 1:

Top foregut-specific and hindgut-specific tag sequences. This table shows the top 25 foregut specific and top 20 hindgut specific tag sequences with the counts and gene annotation.

Format: XLS Size: 32KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Tag sequences enriched in the endoderm SAGE libraries. This table shows the tag sequences and annotations for transcription factors and signaling pathway components that are enriched in the endoderm with a T/L ration > 0.2.

Format: XLS Size: 30KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Primers used for RT-qPCR. This file contains the primer sequences used for gene validation by quantitative RT-PCR.

Format: PDF Size: 26KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 4:

Primers for amplification of the cDNA fragment for in situ probes. This file contains the primer sequences used to amplify DNA fragments used for in situ hybridization probes.

Format: PDF Size: 64KB Download file

This file can be viewed with: Adobe Acrobat Reader


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