Research article
A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE)
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* Corresponding author: Pamela A Hoodless hoodless@bccrc.ca
1 Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada
2 Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, Canada
3 Department of Medical Genetics, University of British Columbia, Vancouver, Canada
4 Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada
BMC Developmental Biology 2007, 7:92 doi:10.1186/1471-213X-7-92
Published: 2 August 2007Additional files
Additional file 1:
Top foregut-specific and hindgut-specific tag sequences. This table shows the top 25 foregut specific and top 20 hindgut specific tag sequences with the counts and gene annotation.
Format: XLS Size: 32KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 2:
Tag sequences enriched in the endoderm SAGE libraries. This table shows the tag sequences and annotations for transcription factors and signaling pathway components that are enriched in the endoderm with a T/L ration > 0.2.
Format: XLS Size: 30KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 3:
Primers used for RT-qPCR. This file contains the primer sequences used for gene validation by quantitative RT-PCR.
Format: PDF Size: 26KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 4:
Primers for amplification of the cDNA fragment for in situ probes. This file contains the primer sequences used to amplify DNA fragments used for in situ hybridization probes.
Format: PDF Size: 64KB Download file
This file can be viewed with: Adobe Acrobat Reader