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Open Access Research article

Apoptosis in mouse fetal and neonatal oocytes during meiotic prophase one

Fataneh Ghafari1, Carlos G Gutierrez2 and Geraldine M Hartshorne34*

Author Affiliations

1 Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

2 Facultad de Medicina Veterinaria, Universidad Nacional Autonoma de Mexico, DF 04510, Mexico

3 Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry, CV2 2DX, UK

4 Centre for Reproductive Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, CV2 2DX, UK

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BMC Developmental Biology 2007, 7:87  doi:10.1186/1471-213X-7-87

Published: 24 July 2007

Abstract

Background

The vast majority of oocytes formed in the fetal ovary do not survive beyond birth. Possible reasons for their loss include the elimination of non-viable genetic constitutions arising through meiosis, however, the precise relationship between meiotic stages and prenatal apoptosis of oocytes remains elusive. We studied oocytes in mouse fetal and neonatal ovaries, 14.5–21 days post coitum, to examine the relationship between oocyte development and programmed cell death during meiotic prophase I.

Results

Microspreads of fetal and neonatal ovarian cells underwent immunocytochemistry for meiosis- and apoptosis-related markers. COR-1 (meiosis-specific) highlighted axial elements of the synaptonemal complex and allowed definitive identification of the stages of meiotic prophase I. Labelling for cleaved poly-(ADP-ribose) polymerase (PARP-1), an inactivated DNA repair protein, indicated apoptosis. The same oocytes were then labelled for DNA double strand breaks (DSBs) using TUNEL. 1960 oocytes produced analysable results.

Oocytes at all stages of meiotic prophase I stained for cleaved PARP-1 and/or TUNEL, or neither. Oocytes with fragmented (19.8%) or compressed (21.2%) axial elements showed slight but significant differences in staining for cleaved PARP-1 and TUNEL to those with intact elements. However, fragmentation of axial elements alone was not a good indicator of cell demise. Cleaved PARP-1 and TUNEL staining were not necessarily coincident, showing that TUNEL is not a reliable marker of apoptosis in oocytes.

Conclusion

Our data indicate that apoptosis can occur throughout meiotic prophase I in mouse fetal and early postnatal oocytes, with greatest incidence at the diplotene stage. Careful selection of appropriate markers for oocyte apoptosis is essential.