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Assets and limitation of in situ electroporation and retroviral transduction to target YS blood islands precursors |
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| In situ electroporation |
Retroviral transduction |
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| Asset |
Limitation |
Asset |
Limitation |
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| 7–7.5 dpc: Mesodermal/pre-hematopoietic precursors |
- Transient - Layer specific delivery - Spatial control of delivery |
- Reduced number of precursors available for transduction |
- Causes extensive cell death - Refractory to transduction ? |
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| Application |
Only possible method |
Unsuitable at this development stage |
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| From 7.5–8 dpc: Hematopoietic precursors |
- Transient - Layer specific delivery - Spatial control of delivery |
- Only a fraction of the YS blood islands is targeted |
- Easy - All hematopoietic precursors may be transduced |
- Does not allow layer specific targeting |
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| Application |
-Transient transgene delivery |
- Sustained expression of the transgene |
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| - Possible layer specific delivery. |
- Possible delivery of ShRNA |
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| - Possible delivery of SiRNA or proteins |
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Hematopoietic precursor frequency within whole control YS (electroporated without construct) and GFP+ cells sorted from electroporated YS-explants were determined through limiting dilution assay. | ||||
Giroux et al. BMC Developmental Biology 2007 7:79 doi:10.1186/1471-213X-7-79 |
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