|
In situelectroporation efficiently delivers the construct to the blood islands |
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| Frequency of recovered hematopoietic precursors |
Number of recovered hematopoietic precursors per YS |
Number of experiments |
|
|
|
|||
| Whole OrgD3-YS |
1/475 |
10–11 |
4 |
| Whole OrgD3-YS electroporated without plasmid |
1/453 |
10 |
3 |
| OrgD3-YS GFP+cells |
1/150 (± 57.62) |
0.56 ± 0.18 per 0.5 YS (targeted area) |
5 |
|
Hematopoietic precursor frequency within whole control YS (electroporated without construct) and GFP+ cells sorted from electroporated YS-explants were determined through limiting dilution assay. | |||
Giroux et al. BMC Developmental Biology 2007 7:79 doi:10.1186/1471-213X-7-79 |
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