Rescue of wing defects. A) Calculation of RNAi-effects within the posterior compartment. Two examples are shown, a control wing (upper) and a mutant wing (lower). Anterior (F1, pink) and posterior (F2, yellow) compartment area (in pixels) was measured as depicted. The area between L3 and L4 was ignored since the antero-posterior boundary cannot be exactly determined. The F2/F1 ratio was calculated for each wing independently to avoid influence of natural variation on wing size e.g. due to nutritional conditions. B) PKD-RNAi was induced in the posterior compartment at 29°C or 25°C as indicated; concurrently, PKD-WT10-2 or DIAP1 were overexpressed. A minimum of five wings of each genotype was measured as depicted in a) and the mean value of F2/F1 was determined. The wild type ratio was taken as 100% and the others calculated as percentage of wild type. Error bars represent standard deviation.
Maier et al. BMC Developmental Biology 2007 7:74 doi:10.1186/1471-213X-7-74