Identification and expression analysis of genes associated with bovine blastocyst formation

doi:10.1186/1471-213X-7-64

BMC Developmental Biology

Volume 7

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Goossens K
Van Soom A
Van Poucke M
Vandaele L
Vandesompele J
Van Zeveren A
Peelman LJ

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Goossens K
Van Soom A
Van Poucke M
Vandaele L
Vandesompele J
Van Zeveren A
Peelman LJ
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Research article

Identification and expression analysis of genes associated with bovine blastocyst formation

Karen Goossens¹ , Ann Van Soom² , Mario Van Poucke¹ , Leen Vandaele² , Jo Vandesompele³ , Alex Van Zeveren¹ and Luc J Peelman¹

¹Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820 Merelbeke, Belgium

²Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium

³Centre for Medical Genetics Ghent, Ghent University Hospital, Medical Research Building, De Pintelaan 185, B-9000 Ghent, Belgium

author email corresponding author email

BMC Developmental Biology 2007, 7:64doi:10.1186/1471-213X-7-64


Published:	8 June 2007

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Additional File 1:

Negative and positive controls for immunofluorescent labelling experiments. Negative (by replacing the primary antibody with goat serum) and double negative controls (only PI staining) were performed to check for non-specific binding of the secondary antibody and for auto-fluorescence. A monolayer of cultured cumulus cells was used as a positive control. (A: negative control for KRT18, B: double negative control for KRT18, C: positive control for KRT18; A': negative control for FN1, B': double negative control for FN1, C': positive control for FN1; A": negative control for MYL6, B": double negative control for MYL6, C": positive control for MYL6).

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