Additional file 4.

Wnt signal does not contribute to the defect of intestine in the absence of pRb. Intestines derived from RB^+/+, RB^+/- and RB^-/- newborn pups were subjected to RT-PCR amplification for the detection of Wnt1, Wnt2, Frizzled1, Frizzled3, DKK and actin used as a control (A) as well as TCF-4 and LEF1 (D). The embryonic intestine sections (E17.5) derived from RB^+/- (left) or RB^-/- (right) mice were stained by IHC with β-catenin (B). Representative sections from RB^+/+, RB^+/- and RB^-/- newborn pups were stained with immunofluoresence by using anti- β-catenin (Green) and DAPI (Blue) (C).

Format: PDF Size: 1.1MB Download file

This file can be viewed with: Adobe Acrobat Reader

Yang and Hinds BMC Developmental Biology 2007 7:6   doi:10.1186/1471-213X-7-6